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@article{1369966,
author = {Adámik, Matej and Kejnovská, Iva and Bažantová, Pavla and Petr, Marek and Renčiuk, Daniel and Vorlíčková, Michaela and Brázdová, Marie and Švarcová, Monika},
article_location = {Paris},
doi = {http://dx.doi.org/10.1016/j.biochi.2016.07.004},
keywords = {G-quadruplex; p53 protein; Telomere DNA; DNA-protein interaction},
language = {eng},
issn = {0300-9084},
journal = {Biochimie},
title = {p53 binds human telomeric G-quadruplex in vitro},
url = {http://dx.doi.org/10.1016/j.biochi.2016.07.004},
volume = {128},
year = {2016}
}
TY - JOUR
ID - 1369966
AU - Adámik, Matej - Kejnovská, Iva - Bažantová, Pavla - Petr, Marek - Renčiuk, Daniel - Vorlíčková, Michaela - Brázdová, Marie - Švarcová, Monika
PY - 2016
TI - p53 binds human telomeric G-quadruplex in vitro
JF - Biochimie
VL - 128
SP - 83-91
EP - 83-91
PB - Elsevier France- editions Scientifiques Medicales Elsevier
SN - 03009084
KW - G-quadruplex
KW - p53 protein
KW - Telomere DNA
KW - DNA-protein interaction
UR - http://dx.doi.org/10.1016/j.biochi.2016.07.004
L2 - http://dx.doi.org/10.1016/j.biochi.2016.07.004
N2 - The tumor suppressor protein p53 is a key factor in genome stability and one of the most studied of DNA binding proteins. This is the first study on the interaction of wild-type p53 with guanine quadruplexes formed by the human telomere sequence. Using electromobility shift assay and ELISA, we show that p53 binding to telomeric G-quadruplexes increases with the number of telomeric repeats. Further, p53 strongly favors G-quadruplexes folded in potassium over those formed in sodium, thus indicating the telomeric G-quadruplex conformational selectivity of p53. The presence of the quadruplex-stabilizing ligand, N-methyl mesoporphyrin IX (NMM), increases p53 recognition of G-quadruplexes in potassium. Using deletion mutants and selective p53 core domain oxidation, both p53 DNA binding domains are shown to be crucial for telomeric G-quadruplex recognition. (C) 2016 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
ER -
ADÁMIK, Matej, Iva KEJNOVSKÁ, Pavla BAŽANTOVÁ, Marek PETR, Daniel RENČIUK, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ and Monika ŠVARCOVÁ. p53 binds human telomeric G-quadruplex in vitro. \textit{Biochimie}. Paris: Elsevier France- editions Scientifiques Medicales Elsevier, vol.~128, p.~83-91. ISSN~0300-9084. doi:10.1016/j.biochi.2016.07.004. 2016.
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