PETR, Marek, Robert HELMA, Alena POLÁŠKOVÁ, Aneta KREJČÍ, Zuzana DVOŘÁKOVÁ, Iva KEJNOVSKÁ, Lucie NAVRÁTILOVÁ, Matej ADÁMIK, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ and Gabriela RUMÍŠKOVÁ. Wild-type p53 binds to MYC promoter G-quadruplex. Bioscience Reports. London: Portland Press LTD, 2016, vol. 36, 11 pp. ISSN 0144-8463. doi:10.1042/BSR20160232.
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Basic information
Original name Wild-type p53 binds to MYC promoter G-quadruplex
Authors PETR, Marek, Robert HELMA, Alena POLÁŠKOVÁ, Aneta KREJČÍ, Zuzana DVOŘÁKOVÁ, Iva KEJNOVSKÁ, Lucie NAVRÁTILOVÁ, Matej ADÁMIK, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ and Gabriela RUMÍŠKOVÁ.
Edition Bioscience Reports, London, Portland Press LTD, 2016, 0144-8463.
Other information
Original language English
Type of outcome Article in a journal
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.906
Doi http://dx.doi.org/10.1042/BSR20160232
UT WoS 000388364900021
Keywords in English DNA-protein interaction; G-quadruplex; MYC; p53 protein
Tags International impact, Reviewed
Changed by Changed by: Mgr. Zuzana Dvořáková, Ph.D., učo 211080. Changed: 17/10/2017 16:38.
Abstract
G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III1 region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.
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