J 2016

Histopathology Image Analysis in Two Long-Term Animal Experiments with Helical Flow Total Artificial Heart

WOTKE, Jiří, Pavel HOMOLKA, Jaromír VASKU, Petr DOBŠÁK, Petra PALANOVÁ et. al.

Basic information

Original name

Histopathology Image Analysis in Two Long-Term Animal Experiments with Helical Flow Total Artificial Heart

Authors

WOTKE, Jiří (203 Czech Republic, belonging to the institution), Pavel HOMOLKA (203 Czech Republic, belonging to the institution), Jaromír VASKU (203 Czech Republic), Petr DOBŠÁK (203 Czech Republic, guarantor, belonging to the institution), Petra PALANOVÁ (203 Czech Republic, belonging to the institution), Veronika MRKVICOVÁ (203 Czech Republic, belonging to the institution), Petr KONEČNÝ (203 Czech Republic, belonging to the institution), Vladimír SOŠKA (203 Czech Republic, belonging to the institution), Michal POHANKA (203 Czech Republic, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Terumi YURIMOTO (392 Japan), Itsuro SAITO (392 Japan), Yusuke INOUE (392 Japan), Takashi ISOYAMA (392 Japan) and Yusuke ABE (392 Japan)

Edition

Artificial organs, Hoboken, Wiley-Blackwell, 2016, 0160-564X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.403

RIV identification code

RIV/00216224:14110/16:00093337

Organization unit

Faculty of Medicine

DOI

UT WoS

000389465300012

Keywords in English

Total artificial heart; Helical flow; Titanium alloy; Microparticles; Histopathology

Tags

Tags

International impact, Reviewed
Změněno: 14/11/2017 10:43, Soňa Böhmová

Abstract

V originále

Histopathological analysis can provide important information in long-term experiments with total artificial heart (TAH). Recently, a new type of blood pump, the helical flow total artificial heart (HF-TAH) was developed. This study aimed to investigate the changes in selected vital organs in animal experiments with implanted HF-TAH. Samples from lung, liver, and kidneys from two female goats (No. 1301 and No. 1304) with implanted HF-TAH were analyzed. Tissue samples were fixed in 10% formaldehyde and 4 mm thick transverse sections were stained with hematoxylin-eosin (HE). Additional staining was done for detection of connective tissue (Masson-Goldner stain) and for detection of iron (hemosiderin) deposits (Perls stain). Sections were scanned at 1003 and 5003 magnification with a light microscope. Experiment no. 1301 survived 100 days (cause of termination was heavy damage of the right pump); experimental goat no. 1304 survived 68 days and was sacrificed due to severe right hydrodynamic bearing malfunction. Histopathological analysis of liver samples proved signs of chronic venostasis with limited focal necrotic zones. Dilated tubules, proteinaceous material in tubular lumen, and hemosiderin deposits were detected in kidney samples. Contamination of the organs by embolized micro-particles was suspected at the autopsy after discovery of visible damage (scratches) of the pump impeller surface (made from titanium alloy) in both experiments. Sporadic deposits of foreign micro-particles (presumably titanium) were observed in most of the analyzed parenchymal organs. However, the described deposits were not in direct connection with inflammatory reactions in the analyzed tissues. Histopathological analysis showed the presence of minimal contamination of the lung, kidney, and liver tissue samples by foreign material (titanium very likely). The analysis showed only limited pathological changes, especially in liver and kidneys, which might be attributed to the influence of artificial perfusion often observed in chronic TAH experiments.