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@article{1371298,
author = {Bagherpoor, Alireza Jian and Doležalová, Dáša and Bárta, Tomáš and Kučírek, Martin and Sani, Soodabeh Abbasi and Ešner, Milan and Bosáková, Michaela and Vinarský, Vladimír and Pešková, Lucie and Hampl, Aleš and Štros, Michal},
article_location = {New Rochelle},
article_number = {5},
doi = {http://dx.doi.org/10.1089/scd.2016.0274},
keywords = {human embryonic stem cells; HMGB1; HMGB2; neuroectodermal cells; differentiation},
language = {eng},
issn = {1547-3287},
journal = {Stem Cells and Development},
title = {Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins},
volume = {26},
year = {2017}
}
TY - JOUR
ID - 1371298
AU - Bagherpoor, Alireza Jian - Doležalová, Dáša - Bárta, Tomáš - Kučírek, Martin - Sani, Soodabeh Abbasi - Ešner, Milan - Bosáková, Michaela - Vinarský, Vladimír - Pešková, Lucie - Hampl, Aleš - Štros, Michal
PY - 2017
TI - Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins
JF - Stem Cells and Development
VL - 26
IS - 5
SP - 328-340
EP - 328-340
PB - Mary Ann Liebert
SN - 15473287
KW - human embryonic stem cells
KW - HMGB1
KW - HMGB2
KW - neuroectodermal cells
KW - differentiation
N2 - HMGB1 and HMGB2 proteins have been implicated in numerous cellular processes, including proliferation, differentiation, apoptosis, and tumor growth. It is unknown whether they are involved in regulating the typical functions of pluripotent human embryonic stem cells (hESCs) and/or those of the differentiated derivatives of hESCs. Using inducible, stably transfected hESCs capable of shRNA-mediated knockdown of HMGB1 and HMGB2, we provide evidence that downregulation of HMGB1 and/or HMGB2 in undifferentiated hESCs does not affect the stemness of cells and induces only minor changes to the proliferation rate, cell-cycle profile, and apoptosis. After differentiation is induced, however, the downregulation of those proteins has important effects on proliferation, apoptosis, telomerase activity, and the efficiency of differentiation toward the neuroectodermal lineage. Furthermore, those processes are affected only when one, but not both, of the two proteins is downregulated; the knockdown of both HMGB1 and HMGB2 results in a normal phenotype. Those results advance our knowledge of regulation of hESC and human neuroectodermal cell differentiation and illustrate the distinct roles of HMGB1 and HMGB2 during early human development.
ER -
BAGHERPOOR, Alireza Jian, Dáša DOLEŽALOVÁ, Tomáš BÁRTA, Martin KUČÍREK, Soodabeh Abbasi SANI, Milan EŠNER, Michaela BOSÁKOVÁ, Vladimír VINARSKÝ, Lucie PEŠKOVÁ, Aleš HAMPL and Michal ŠTROS. Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins. \textit{Stem Cells and Development}. New Rochelle: Mary Ann Liebert, 2017, vol.~26, No~5, p.~328-340. ISSN~1547-3287. Available from: https://dx.doi.org/10.1089/scd.2016.0274.
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