Properties of Human Embryonic Stem Cells and Their
Differentiated Derivatives Depend on Nonhistone DNA-Binding
HMGB1 and HMGB2 Proteins

J 2017

Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins

BAGHERPOOR, Alireza Jian, Dáša DOLEŽALOVÁ, Tomáš BÁRTA, Martin KUČÍREK, Soodabeh Abbasi SANI et. al.

Basic information

Original name

Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins

Authors

BAGHERPOOR, Alireza Jian (203 Czech Republic), Dáša DOLEŽALOVÁ (703 Slovakia, guarantor, belonging to the institution), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Martin KUČÍREK (203 Czech Republic), Soodabeh Abbasi SANI (203 Czech Republic), Milan EŠNER (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Vladimír VINARSKÝ (203 Czech Republic), Lucie PEŠKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Michal ŠTROS (203 Czech Republic)

Edition

Stem Cells and Development, New Rochelle, Mary Ann Liebert, 2017, 1547-3287

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.315

RIV identification code

RIV/00216224:14110/17:00094624

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1089/scd.2016.0274

UT WoS

000394588800003

Keywords in English

human embryonic stem cells; HMGB1; HMGB2; neuroectodermal cells; differentiation

Abstract

V originále

HMGB1 and HMGB2 proteins have been implicated in numerous cellular processes, including proliferation, differentiation, apoptosis, and tumor growth. It is unknown whether they are involved in regulating the typical functions of pluripotent human embryonic stem cells (hESCs) and/or those of the differentiated derivatives of hESCs. Using inducible, stably transfected hESCs capable of shRNA-mediated knockdown of HMGB1 and HMGB2, we provide evidence that downregulation of HMGB1 and/or HMGB2 in undifferentiated hESCs does not affect the stemness of cells and induces only minor changes to the proliferation rate, cell-cycle profile, and apoptosis. After differentiation is induced, however, the downregulation of those proteins has important effects on proliferation, apoptosis, telomerase activity, and the efficiency of differentiation toward the neuroectodermal lineage. Furthermore, those processes are affected only when one, but not both, of the two proteins is downregulated; the knockdown of both HMGB1 and HMGB2 results in a normal phenotype. Those results advance our knowledge of regulation of hESC and human neuroectodermal cell differentiation and illustrate the distinct roles of HMGB1 and HMGB2 during early human development.

Links

GAP305/12/2475, research and development project

Name: Proteiny HMGB: funkce v biologii telomer a lidských embryonálních kmenových buněk

Investor: Czech Science Foundation

GA15-23033S, research and development project

Name: Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk

Investor: Czech Science Foundation

GJ15-18316Y, research and development project

Name: Úloha microRNA v řízení buněčného dělení a diferenciace lidských embryonálních kmenových buněk do neurálních kmenových buněk. (Acronym: miRNA v diferenciaci lidských EK buněk)

Investor: Czech Science Foundation

GJ16-24004Y, research and development project

Name: Indukce buněčné plasticity prostřednictvím modulace mikroRNA molekul: Nový přístup pro přeprogramování buněk

Investor: Czech Science Foundation

MUNI/A/1352/2015, interní kód MU

Name: Zdroje pro tkáňové inženýrství 6 (Acronym: TissueENG 6)

Investor: Masaryk University, Category A

Citovat

BAGHERPOOR, Alireza Jian, Dáša DOLEŽALOVÁ, Tomáš BÁRTA, Martin KUČÍREK, Soodabeh Abbasi SANI, Milan EŠNER, Michaela BOSÁKOVÁ, Vladimír VINARSKÝ, Lucie PEŠKOVÁ, Aleš HAMPL and Michal ŠTROS. Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells and Development. New Rochelle: Mary Ann Liebert, 2017, vol. 26, No 5, p. 328-340. ISSN 1547-3287. Available from: https://dx.doi.org/10.1089/scd.2016.0274.

@article{1371298,
   author = {Bagherpoor, Alireza Jian and Doležalová, Dáša and Bárta, Tomáš and Kučírek, Martin and Sani, Soodabeh Abbasi and Ešner, Milan and Bosáková, Michaela and Vinarský, Vladimír and Pešková, Lucie and Hampl, Aleš and Štros, Michal},
   article_location = {New Rochelle},
   article_number = {5},
   doi = {http://dx.doi.org/10.1089/scd.2016.0274},
   keywords = {human embryonic stem cells; HMGB1; HMGB2; neuroectodermal cells; differentiation},
   language = {eng},
   issn = {1547-3287},
   journal = {Stem Cells and Development},
   title = {Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins},
   volume = {26},
   year = {2017}
}

TY  - JOUR
ID  - 1371298
AU  - Bagherpoor, Alireza Jian - Doležalová, Dáša - Bárta, Tomáš - Kučírek, Martin - Sani, Soodabeh Abbasi - Ešner, Milan - Bosáková, Michaela - Vinarský, Vladimír - Pešková, Lucie - Hampl, Aleš - Štros, Michal
PY  - 2017
TI  - Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins
JF  - Stem Cells and Development
VL  - 26
IS  - 5
SP  - 328-340
EP  - 328-340
PB  - Mary Ann Liebert
SN  - 15473287
KW  - human embryonic stem cells
KW  - HMGB1
KW  - HMGB2
KW  - neuroectodermal cells
KW  - differentiation
N2  - HMGB1 and HMGB2 proteins have been implicated in numerous cellular processes, including proliferation, differentiation, apoptosis, and tumor growth. It is unknown whether they are involved in regulating the typical functions of pluripotent human embryonic stem cells (hESCs) and/or those of the differentiated derivatives of hESCs. Using inducible, stably transfected hESCs capable of shRNA-mediated knockdown of HMGB1 and HMGB2, we provide evidence that downregulation of HMGB1 and/or HMGB2 in undifferentiated hESCs does not affect the stemness of cells and induces only minor changes to the proliferation rate, cell-cycle profile, and apoptosis. After differentiation is induced, however, the downregulation of those proteins has important effects on proliferation, apoptosis, telomerase activity, and the efficiency of differentiation toward the neuroectodermal lineage. Furthermore, those processes are affected only when one, but not both, of the two proteins is downregulated; the knockdown of both HMGB1 and HMGB2 results in a normal phenotype. Those results advance our knowledge of regulation of hESC and human neuroectodermal cell differentiation and illustrate the distinct roles of HMGB1 and HMGB2 during early human development.
ER  -

BAGHERPOOR, Alireza Jian, Dáša DOLEŽALOVÁ, Tomáš BÁRTA, Martin KUČÍREK, Soodabeh Abbasi SANI, Milan EŠNER, Michaela BOSÁKOVÁ, Vladimír VINARSKÝ, Lucie PEŠKOVÁ, Aleš HAMPL and Michal ŠTROS. Properties of Human Embryonic Stem Cells and Their Differentiated Derivatives Depend on Nonhistone DNA-Binding HMGB1 and HMGB2 Proteins. \textit{Stem Cells and Development}. New Rochelle: Mary Ann Liebert, 2017, vol.~26, No~5, p.~328-340. ISSN~1547-3287. Available from: https://dx.doi.org/10.1089/scd.2016.0274.

Detailed Information on Publication Record