Spatial mapping of metals in tissue-sections using combination
of mass-spectrometry and histology through image registration

J 2017

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

ANYZ, Jiri; Lenka VYSLOUZILOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Viktor KANICKÝ et. al.

Základní údaje

Originální název

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Autoři

ANYZ, Jiri; Lenka VYSLOUZILOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Viktor KANICKÝ; Hajo HAASE; Vratislav HORAK; Olga STEPANKOVA; Zbyněk HEGER a Vojtěch ADAM

Vydání

Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2017, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.122

Kód RIV

RIV/00216224:14310/17:00096114

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1038/srep40169

UT WoS

000392234200001

EID Scopus

2-s2.0-85009230317

Klíčová slova anglicky

Bioanalytical chemistry; melanoma

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2018 22:52, Ing. Nicole Zrilić

Anotace

V originále

We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ANYZ, Jiri; Lenka VYSLOUZILOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Viktor KANICKÝ; Hajo HAASE; Vratislav HORAK; Olga STEPANKOVA; Zbyněk HEGER a Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2017, roč. 7, January, s. nestránkováno, 13 s. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/srep40169.

@article{1371609,
   author = {Anyz, Jiri and Vyslouzilova, Lenka and Vaculovič, Tomáš and Tvrdoňová, Michaela and Kanický, Viktor and Haase, Hajo and Horak, Vratislav and Stepankova, Olga and Heger, Zbyněk and Adam, Vojtěch},
   article_location = {LONDON},
   article_number = {January},
   doi = {https://doi.org/10.1038/srep40169},
   keywords = {Bioanalytical chemistry; melanoma},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration},
   url = {http://www.nature.com/articles/srep40169},
   volume = {7},
   year = {2017}
}

TY  - JOUR
ID  - 1371609
AU  - Anyz, Jiri - Vyslouzilova, Lenka - Vaculovič, Tomáš - Tvrdoňová, Michaela - Kanický, Viktor - Haase, Hajo - Horak, Vratislav - Stepankova, Olga - Heger, Zbyněk - Adam, Vojtěch
PY  - 2017
TI  - Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration
JF  - Scientific Reports
VL  - 7
IS  - January
SP  - nestránkováno
EP  - nestránkováno
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Bioanalytical chemistry
KW  - melanoma
UR  - http://www.nature.com/articles/srep40169
L2  - http://www.nature.com/articles/srep40169
N2  - We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
ER  -

ANYZ, Jiri; Lenka VYSLOUZILOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Viktor KANICKÝ; Hajo HAASE; Vratislav HORAK; Olga STEPANKOVA; Zbyněk HEGER a Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2017, roč.~7, January, s.~nestránkováno, 13 s. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/srep40169.

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