| ANYZ, Jiri, Lenka VYSLOUZILOVA, Tomáš VACULOVIČ, Michaela TVRDOŇOVÁ, Viktor KANICKÝ, Hajo HAASE, Vratislav HORAK, Olga STEPANKOVA, Zbyněk HEGER a Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, roč. 7, January, s. nestránkováno, 13 s. ISSN 2045-2322. doi:10.1038/srep40169. 2017. |
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@article{1371609,
author = {Anyz, Jiri and Vyslouzilova, Lenka and Vaculovič, Tomáš and Tvrdoňová, Michaela and Kanický, Viktor and Haase, Hajo and Horak, Vratislav and Stepankova, Olga and Heger, Zbyněk and Adam, Vojtěch},
article_location = {LONDON},
article_number = {January},
doi = {http://dx.doi.org/10.1038/srep40169},
keywords = {Bioanalytical chemistry; melanoma},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration},
url = {http://www.nature.com/articles/srep40169},
volume = {7},
year = {2017}
}
TY - JOUR
ID - 1371609
AU - Anyz, Jiri - Vyslouzilova, Lenka - Vaculovič, Tomáš - Tvrdoňová, Michaela - Kanický, Viktor - Haase, Hajo - Horak, Vratislav - Stepankova, Olga - Heger, Zbyněk - Adam, Vojtěch
PY - 2017
TI - Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration
JF - Scientific Reports
VL - 7
IS - January
SP - nestránkováno
EP - nestránkováno
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - Bioanalytical chemistry
KW - melanoma
UR - http://www.nature.com/articles/srep40169
L2 - http://www.nature.com/articles/srep40169
N2 - We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
ER -
ANYZ, Jiri, Lenka VYSLOUZILOVA, Tomáš VACULOVIČ, Michaela TVRDOŇOVÁ, Viktor KANICKÝ, Hajo HAASE, Vratislav HORAK, Olga STEPANKOVA, Zbyněk HEGER a Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, roč.~7, January, s.~nestránkováno, 13 s. ISSN~2045-2322. doi:10.1038/srep40169. 2017.
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