ANYZ, Jiri, Lenka VYSLOUZILOVA, Tomáš VACULOVIČ, Michaela TVRDOŇOVÁ, Viktor KANICKÝ, Hajo HAASE, Vratislav HORAK, Olga STEPANKOVA, Zbyněk HEGER and Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2017, vol. 7, January, p. nestránkováno, 13 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep40169. |
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@article{1371609, author = {Anyz, Jiri and Vyslouzilova, Lenka and Vaculovič, Tomáš and Tvrdoňová, Michaela and Kanický, Viktor and Haase, Hajo and Horak, Vratislav and Stepankova, Olga and Heger, Zbyněk and Adam, Vojtěch}, article_location = {LONDON}, article_number = {January}, doi = {http://dx.doi.org/10.1038/srep40169}, keywords = {Bioanalytical chemistry; melanoma}, language = {eng}, issn = {2045-2322}, journal = {Scientific Reports}, title = {Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration}, url = {http://www.nature.com/articles/srep40169}, volume = {7}, year = {2017} }
TY - JOUR ID - 1371609 AU - Anyz, Jiri - Vyslouzilova, Lenka - Vaculovič, Tomáš - Tvrdoňová, Michaela - Kanický, Viktor - Haase, Hajo - Horak, Vratislav - Stepankova, Olga - Heger, Zbyněk - Adam, Vojtěch PY - 2017 TI - Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration JF - Scientific Reports VL - 7 IS - January SP - nestránkováno EP - nestránkováno PB - NATURE PUBLISHING GROUP SN - 20452322 KW - Bioanalytical chemistry KW - melanoma UR - http://www.nature.com/articles/srep40169 L2 - http://www.nature.com/articles/srep40169 N2 - We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer. ER -
ANYZ, Jiri, Lenka VYSLOUZILOVA, Tomáš VACULOVIČ, Michaela TVRDOŇOVÁ, Viktor KANICKÝ, Hajo HAASE, Vratislav HORAK, Olga STEPANKOVA, Zbyněk HEGER and Vojtěch ADAM. Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2017, vol.~7, January, p.~nestránkováno, 13 pp. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/srep40169.
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