Detailed Information on Publication Record
2017
A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations
ABDEL-MOHSEN, A.M., Josef JANČÁŘ, R.M. ABDEL-RAHMAN, Libor VOJTEK, Pavel HYRŠL et. al.Basic information
Original name
A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations
Name in Czech
A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations
Authors
ABDEL-MOHSEN, A.M. (818 Egypt, guarantor), Josef JANČÁŘ (203 Czech Republic), R.M. ABDEL-RAHMAN (818 Egypt), Libor VOJTEK (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution), Monika DUŠKOVÁ (203 Czech Republic, belonging to the institution) and Helena NEJEZCHLEBOVÁ (203 Czech Republic)
Edition
International Journal of Pharmaceutics, Amsterdam, Elsevier Science BV, 2017, 0378-5173
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30102 Immunology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.862
RIV identification code
RIV/00216224:14310/17:00096115
Organization unit
Faculty of Science
UT WoS
000396948400025
Keywords (in Czech)
In-situ stříbro; hyaluronan; cytotoxicita; obvazový materiál
Keywords in English
In-situ silver; hyaluronan; cytotoxicity; wound dressing
Tags
International impact, Reviewed
Změněno: 27/3/2018 14:47, Ing. Nicole Zrilić
Abstract
V originále
In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA 3 fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.