Detailed Information on Publication Record
2017
Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis
ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Rafael PELÁEZ et. al.Basic information
Original name
Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis
Authors
ANGUIANO, María (724 Spain), Carlos CASTILLA (724 Spain), Martin MAŠKA (203 Czech Republic, guarantor, belonging to the institution), Cristina EDERRA (724 Spain), Rafael PELÁEZ (724 Spain), Xabier MORALES (724 Spain), Gorka MUÑOZ-ARRIETA (724 Spain), Maite MUJIKA (724 Spain), Michal KOZUBEK (203 Czech Republic, belonging to the institution), Arrate MUÑOZ-BARRUTIA (724 Spain), Ana ROUZAUT (724 Spain), Sergio ARANA (724 Spain), José Manuel GARCIA-AZNAR (724 Spain) and Carlos ORTIZ-DE-SOLORZANO (724 Spain)
Edition
PLoS ONE, 2017, 1932-6203
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher
Hungary
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.766
RIV identification code
RIV/00216224:14330/17:00094626
Organization unit
Faculty of Informatics
UT WoS
000393700100040
Keywords in English
cancer; cell migration; basement membrane; cancer invasion; hydrogel; Matrigel; microfluidic devices; cell tracking; mechanobiology
Tags
Tags
International impact, Reviewed
Změněno: 14/6/2022 12:28, RNDr. Pavel Šmerk, Ph.D.
Abstract
V originále
Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments. We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions. Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue. Our quantification of migration speed confirms a biphasic role of Matrigel. At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment. At high concentration, Matrigel slows down migration, possibly due excessive attachment. Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix. In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions. This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
Links
| GBP302/12/G157, research and development project |
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