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@article{1371977,
author = {Všianská, Pavla and Bezděková, Renata and Kryukov, Fedor and Almáši, Martina and Pour, Luděk and Penka, Miroslav and Hájek, Roman and Říhová, Lucie},
article_location = {Hoboken},
article_number = {1},
doi = {http://dx.doi.org/10.1111/ejh.12779},
keywords = {aldehyde dehydrogenase; B-cell; multiple myeloma; plasma cell},
language = {eng},
issn = {0902-4441},
journal = {European Journal of Haematology},
title = {Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors},
url = {http://dx.doi.org/10.1111/ejh.12779},
volume = {98},
year = {2017}
}
TY - JOUR
ID - 1371977
AU - Všianská, Pavla - Bezděková, Renata - Kryukov, Fedor - Almáši, Martina - Pour, Luděk - Penka, Miroslav - Hájek, Roman - Říhová, Lucie
PY - 2017
TI - Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
JF - European Journal of Haematology
VL - 98
IS - 1
SP - 19-25
EP - 19-25
PB - Wiley-Blackwell
SN - 09024441
KW - aldehyde dehydrogenase
KW - B-cell
KW - multiple myeloma
KW - plasma cell
UR - http://dx.doi.org/10.1111/ejh.12779
L2 - http://dx.doi.org/10.1111/ejh.12779
N2 - Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myeloma-initiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19-PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19+PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
ER -
VŠIANSKÁ, Pavla, Renata BEZDĚKOVÁ, Fedor KRYUKOV, Martina ALMÁŠI, Luděk POUR, Miroslav PENKA, Roman HÁJEK a Lucie ŘÍHOVÁ. Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors. \textit{European Journal of Haematology}. Hoboken: Wiley-Blackwell, roč.~98, č.~1, s.~19-25. ISSN~0902-4441. doi:10.1111/ejh.12779. 2017.
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