Activity of aldehyde dehydrogenase in B-cell and plasma cell
subsets of monoclonal gammopathy patients and healthy donors

J 2017

Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors

VŠIANSKÁ, Pavla, Renata BEZDĚKOVÁ, Fedor KRYUKOV, Martina ALMÁŠI, Luděk POUR et. al.

Basic information

Original name

Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors

Authors

VŠIANSKÁ, Pavla, Renata BEZDĚKOVÁ, Fedor KRYUKOV, Martina ALMÁŠI, Luděk POUR, Miroslav PENKA, Roman HÁJEK and Lucie ŘÍHOVÁ

Edition

European Journal of Haematology, Hoboken, Wiley-Blackwell, 2017, 0902-4441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.595

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/ejh.12779

UT WoS

000393166600002

Keywords in English

aldehyde dehydrogenase; B-cell; multiple myeloma; plasma cell

Abstract

V originále

Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myeloma-initiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19-PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19+PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.

Links

NV15-29508A, research and development project

Name: Cirkulující nukleové kyseliny jako markery progrese mnohočetného myelomu

Citovat

VŠIANSKÁ, Pavla, Renata BEZDĚKOVÁ, Fedor KRYUKOV, Martina ALMÁŠI, Luděk POUR, Miroslav PENKA, Roman HÁJEK and Lucie ŘÍHOVÁ. Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors. European Journal of Haematology. Hoboken: Wiley-Blackwell, 2017, vol. 98, No 1, p. 19-25. ISSN 0902-4441. Available from: https://dx.doi.org/10.1111/ejh.12779.

@article{1371977,
   author = {Všianská, Pavla and Bezděková, Renata and Kryukov, Fedor and Almáši, Martina and Pour, Luděk and Penka, Miroslav and Hájek, Roman and Říhová, Lucie},
   article_location = {Hoboken},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/ejh.12779},
   keywords = {aldehyde dehydrogenase; B-cell; multiple myeloma; plasma cell},
   language = {eng},
   issn = {0902-4441},
   journal = {European Journal of Haematology},
   title = {Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors},
   url = {http://dx.doi.org/10.1111/ejh.12779},
   volume = {98},
   year = {2017}
}

TY  - JOUR
ID  - 1371977
AU  - Všianská, Pavla - Bezděková, Renata - Kryukov, Fedor - Almáši, Martina - Pour, Luděk - Penka, Miroslav - Hájek, Roman - Říhová, Lucie
PY  - 2017
TI  - Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
JF  - European Journal of Haematology
VL  - 98
IS  - 1
SP  - 19-25
EP  - 19-25
PB  - Wiley-Blackwell
SN  - 09024441
KW  - aldehyde dehydrogenase
KW  - B-cell
KW  - multiple myeloma
KW  - plasma cell
UR  - http://dx.doi.org/10.1111/ejh.12779
L2  - http://dx.doi.org/10.1111/ejh.12779
N2  - Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myeloma-initiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19-PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19+PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
ER  -

VŠIANSKÁ, Pavla, Renata BEZDĚKOVÁ, Fedor KRYUKOV, Martina ALMÁŠI, Luděk POUR, Miroslav PENKA, Roman HÁJEK and Lucie ŘÍHOVÁ. Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors. \textit{European Journal of Haematology}. Hoboken: Wiley-Blackwell, 2017, vol.~98, No~1, p.~19-25. ISSN~0902-4441. Available from: https://dx.doi.org/10.1111/ejh.12779.

Detailed Information on Publication Record