FANNES, Wouter, Maarten Pieterjan VANHOVE, Tine HUYSE and Giuseppe PALADINI. A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. Parasitology Research. NEW YORK: Springer-Verlag Berlin Heidelberg 2015, 2015, vol. 114, No 5, p. 2031-2034. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4446-7.
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Basic information
Original name A scanning electron microscope technique for studying the sclerites of Cichlidogyrus
Authors FANNES, Wouter (56 Belgium), Maarten Pieterjan VANHOVE (56 Belgium, guarantor, belonging to the institution), Tine HUYSE (56 Belgium) and Giuseppe PALADINI (826 United Kingdom of Great Britain and Northern Ireland).
Edition Parasitology Research, NEW YORK, Springer-Verlag Berlin Heidelberg 2015, 2015, 0932-0113.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.027
RIV identification code RIV/00216224:14310/15:00088664
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00436-015-4446-7
UT WoS 000352723200046
Keywords in English Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Lucie Jarošová, DiS., učo 205746. Changed: 7/3/2018 15:46.
Abstract
The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.
Links
GBP505/12/G112, research and development projectName: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation
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