A scanning electron microscope technique for studying the
sclerites of Cichlidogyrus

J 2015

A scanning electron microscope technique for studying the sclerites of Cichlidogyrus

FANNES, Wouter; Maarten Pieterjan VANHOVE; Tine HUYSE and Giuseppe PALADINI

Basic information

Original name

A scanning electron microscope technique for studying the sclerites of Cichlidogyrus

Authors

FANNES, Wouter (56 Belgium); Maarten Pieterjan VANHOVE (56 Belgium, guarantor, belonging to the institution); Tine HUYSE (56 Belgium) and Giuseppe PALADINI (826 United Kingdom of Great Britain and Northern Ireland)

Edition

Parasitology Research, NEW YORK, Springer-Verlag Berlin Heidelberg 2015, 2015, 0932-0113

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.027

RIV identification code

RIV/00216224:14310/15:00088664

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00436-015-4446-7

UT WoS

000352723200046

EID Scopus

2-s2.0-84937764357

Keywords in English

Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus

Abstract

V originále

The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Cite

FANNES, Wouter; Maarten Pieterjan VANHOVE; Tine HUYSE and Giuseppe PALADINI. A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. Parasitology Research. NEW YORK: Springer-Verlag Berlin Heidelberg 2015, 2015, vol. 114, No 5, p. 2031-2034. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4446-7.

@article{1372035,
   author = {Fannes, Wouter and Vanhove, Maarten Pieterjan and Huyse, Tine and Paladini, Giuseppe},
   article_location = {NEW YORK},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00436-015-4446-7},
   keywords = {Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {A scanning electron microscope technique for studying the sclerites of Cichlidogyrus},
   volume = {114},
   year = {2015}
}

TY  - JOUR
ID  - 1372035
AU  - Fannes, Wouter - Vanhove, Maarten Pieterjan - Huyse, Tine - Paladini, Giuseppe
PY  - 2015
TI  - A scanning electron microscope technique for studying the sclerites of Cichlidogyrus
JF  - Parasitology Research
VL  - 114
IS  - 5
SP  - 2031-2034
EP  - 2031-2034
PB  - Springer-Verlag Berlin Heidelberg 2015
SN  - 09320113
KW  - Platyhelminthes
KW  - Cichlidae
KW  - Anchor
KW  - MCO
KW  - Male apparatus
N2  - The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.
ER  -

FANNES, Wouter; Maarten Pieterjan VANHOVE; Tine HUYSE and Giuseppe PALADINI. A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. \textit{Parasitology Research}. NEW YORK: Springer-Verlag Berlin Heidelberg 2015, 2015, vol.~114, No~5, p.~2031-2034. ISSN~0932-0113. Available from: https://dx.doi.org/10.1007/s00436-015-4446-7.

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