Phytoestrogens in milk: Overestimations caused by contamination
of the hydrolytic enzyme used during sample extraction

J 2016

Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Tereza PROCHÁZKOVÁ, Michaela PRUDÍKOVÁ, Luděk BLÁHA et. al.

Basic information

Original name

Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction

Authors

BLÁHOVÁ, Lucie (203 Czech Republic, belonging to the institution), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution), Tereza PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Michaela PRUDÍKOVÁ (203 Czech Republic) and Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Dairy Science, New York, Elsevier Science Inc. 2016, 0022-0302

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21101 Food and beverages

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.474

RIV identification code

RIV/00216224:14310/16:00093507

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3168/jds.2016-10926

UT WoS

000381326400012

Keywords in English

bovine milk; phytoestrogen; mass spectrometry; equol

Abstract

V originále

Isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. Monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. The efficiency and accuracy of phytoestrogen analyses in complex matrices such as milk depend on the extraction procedure, which often uses hydrolysis by means of the beta-glucuronidase/sulfatase enzyme originating from Helix pomatia. The present study reveals that the commercially available hydrolytic enzyme is contaminated by several phytoestrogen isoflavones (genistein, daidzein, formononetin, and biochanin A) and their metabolite equol, as well as flavones (naringenin and apigenin) and coumestrol. We show that the concentrations of daidzein and genistein in the enzyme could have impaired the results of analyses of the main isoflavones in several previously published studies. Of 8 analyzed compounds, only equol was confirmed in the present study and it serves as a reliable marker of phytoestrogens originating from cow feed. Critical reassessment of phytoestrogen concentrations in milk is needed because several previously published studies might have overestimated the concentrations depending on the extraction procedure used.

Links

LM2011028, research and development project

Name: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Tereza PROCHÁZKOVÁ, Michaela PRUDÍKOVÁ and Luděk BLÁHA. Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction. Journal of Dairy Science. New York: Elsevier Science Inc., 2016, vol. 99, No 9, p. 6973-6982. ISSN 0022-0302. Available from: https://dx.doi.org/10.3168/jds.2016-10926.

@article{1372379,
   author = {Bláhová, Lucie and Kohoutek, Jiří and Procházková, Tereza and Prudíková, Michaela and Bláha, Luděk},
   article_location = {New York},
   article_number = {9},
   doi = {http://dx.doi.org/10.3168/jds.2016-10926},
   keywords = {bovine milk; phytoestrogen; mass spectrometry; equol},
   language = {eng},
   issn = {0022-0302},
   journal = {Journal of Dairy Science},
   title = {Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction},
   url = {http://www.sciencedirect.com/science/article/pii/S0022030216304143},
   volume = {99},
   year = {2016}
}

TY  - JOUR
ID  - 1372379
AU  - Bláhová, Lucie - Kohoutek, Jiří - Procházková, Tereza - Prudíková, Michaela - Bláha, Luděk
PY  - 2016
TI  - Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction
JF  - Journal of Dairy Science
VL  - 99
IS  - 9
SP  - 6973-6982
EP  - 6973-6982
PB  - Elsevier Science Inc.
SN  - 00220302
KW  - bovine milk
KW  - phytoestrogen
KW  - mass spectrometry
KW  - equol
UR  - http://www.sciencedirect.com/science/article/pii/S0022030216304143
L2  - http://www.sciencedirect.com/science/article/pii/S0022030216304143
N2  - Isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. Monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. The efficiency and accuracy of phytoestrogen analyses in complex matrices such as milk depend on the extraction procedure, which often uses hydrolysis by means of the beta-glucuronidase/sulfatase enzyme originating from Helix pomatia. The present study reveals that the commercially available hydrolytic enzyme is contaminated by several phytoestrogen isoflavones (genistein, daidzein, formononetin, and biochanin A) and their metabolite equol, as well as flavones (naringenin and apigenin) and coumestrol. We show that the concentrations of daidzein and genistein in the enzyme could have impaired the results of analyses of the main isoflavones in several previously published studies. Of 8 analyzed compounds, only equol was confirmed in the present study and it serves as a reliable marker of phytoestrogens originating from cow feed. Critical reassessment of phytoestrogen concentrations in milk is needed because several previously published studies might have overestimated the concentrations depending on the extraction procedure used.
ER  -

BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Tereza PROCHÁZKOVÁ, Michaela PRUDÍKOVÁ and Luděk BLÁHA. Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2016, vol.~99, No~9, p.~6973-6982. ISSN~0022-0302. Available from: https://dx.doi.org/10.3168/jds.2016-10926.

Detailed Information on Publication Record