J 2016

Chemopreventive Agents Attenuate Rapid Inhibition of Gap Junctional Intercellular Communication Induced by Environmental Toxicants

BABICA, Pavel, Lucie ČTVERÁČKOVÁ, Zuzana LENČEŠOVÁ, James E. TROSKO, Brad L. UPHAM et. al.

Basic information

Original name

Chemopreventive Agents Attenuate Rapid Inhibition of Gap Junctional Intercellular Communication Induced by Environmental Toxicants

Authors

BABICA, Pavel (203 Czech Republic, guarantor, belonging to the institution), Lucie ČTVERÁČKOVÁ (203 Czech Republic, belonging to the institution), Zuzana LENČEŠOVÁ (203 Czech Republic, belonging to the institution), James E. TROSKO (840 United States of America) and Brad L. UPHAM (840 United States of America)

Edition

Nutrition and cancer : an international journal, ABINGDON, ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD, 2016, 0163-5581

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.447

RIV identification code

RIV/00216224:14310/16:00093527

Organization unit

Faculty of Science

DOI

UT WoS

000380883200013

Keywords in English

CELL-CELL COMMUNICATION; CANCER CHEMOPREVENTION; STEM-CELLS; GREEN TEA; H2O2-INDUCED INHIBITION; CHEMICAL CARCINOGENESIS; DOWN-REGULATION; CHEMOTHERAPY; PREVENTION; QUERCETIN

Tags

Tags

International impact, Reviewed
Změněno: 2/3/2017 12:12, Mgr. Michaela Hylsová, Ph.D.

Abstract

V originále

Altered gap junctional intercellular communication (GJIC) has been associated with chemical carcinogenesis, where both chemical tumor promoters and chemopreventive agents (CPAs) are known to conversely modulate GJIC. The aim of this study was to investigate whether attenuation of chemically inhibited GJIC represents a common outcome induced by different CPAs, which could be effectively evaluated using in vitro methods. Rat liver epithelial cells WB-F344 were pretreated with a CPA for either 30min or 24h, and then exposed to GJIC-inhibiting concentration of a selected tumor promoter or environmental toxicant [12-O-tetradecanoylphorbol-13-acetate (TPA), lindane, fluoranthene, 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), perfluorooctanoic acid (PFOA), or pentachlorophenol]. Out of nine CPAs tested, quercetin and silibinin elicited the most pronounced effects, preventing the dysregulation of GJIC by all the GJIC inhibitors, but DDT. Metformin and curcumin attenuated the effects of three GJIC inhibitors, whereas the other CPAs prevented the effects of two (diallyl sulfide, emodin) or one (indole-3-carbinol, thymoquinone) GJIC inhibitor. Significant attenuation of chemically induced inhibition of GJIC was observed in 27 (50%) out of 54 possible combinations of nine CPAs and six GJIC inhibitors. Our data demonstrate that in vitro evaluation of GJIC can be used as an effective screening tool for identification of chemicals with potential chemopreventive activity.

Links

LM2015051, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR
LO1214, research and development project
Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)
Investor: Ministry of Education, Youth and Sports of the CR