| PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ and Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). Xenobiotica. UK: Taylor & Francis Group, 2016, vol. 46, No 2, p. 132-140. ISSN 0049-8254. Available from: https://dx.doi.org/10.3109/00498254.2015.1060370. |
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@article{1372489,
author = {Prchal, Lukáš and Vokřál, Ivan and Kašný, Martin and Rejšková, Lenka and Zajíčková, Markéta and Lamka, Jiří and Skálová, Lenka and Lecová, Lenka and Szotáková, Barbora},
article_location = {UK},
article_number = {2},
doi = {http://dx.doi.org/10.3109/00498254.2015.1060370},
keywords = {Albendazole; anthelmintics; biotransformation; drug-metabolizing enzymes; mebendazole},
language = {eng},
issn = {0049-8254},
journal = {Xenobiotica},
title = {Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)},
volume = {46},
year = {2016}
}
TY - JOUR
ID - 1372489
AU - Prchal, Lukáš - Vokřál, Ivan - Kašný, Martin - Rejšková, Lenka - Zajíčková, Markéta - Lamka, Jiří - Skálová, Lenka - Lecová, Lenka - Szotáková, Barbora
PY - 2016
TI - Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)
JF - Xenobiotica
VL - 46
IS - 2
SP - 132-140
EP - 132-140
PB - Taylor & Francis Group
SN - 00498254
KW - Albendazole
KW - anthelmintics
KW - biotransformation
KW - drug-metabolizing enzymes
KW - mebendazole
N2 - 1.Giant liver fluke Fascioloides magna is a dangerous parasite, which infects herbivores. It was imported to Europe from North America and started to spread. Benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. However their effect might be altered via drug-metabolizing enzymes of this parasite.2.The aim of our study was to determine the activities of drug-metabolizing enzymes in F. magna and the metabolism of above mentioned anthelmintics.3.Activities of several oxidative, reductive and conjugative enzymes towards various model xenobiotic substrates were found in F. magna subcellular fractions.4.Subcellular fractions from F. magna oxidized albendazole to its sulphoxide metabolite and reduced mebendazole to hydroxyl-mebendazole. Under ex vivo conditions, only very-low concentrations of these compounds were detected using high-performance liquid chromatography/mass spectrometry.5.The results indicate that the giant liver fluke possesses the active xenobiotic-metabolizing system. The overexpression of this system may play an important role in parasite resistance against these anthelmintics.
ER -
PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ and Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). \textit{Xenobiotica}. UK: Taylor \&{} Francis Group, 2016, vol.~46, No~2, p.~132-140. ISSN~0049-8254. Available from: https://dx.doi.org/10.3109/00498254.2015.1060370.
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