Metabolism of drugs and other xenobiotics in giant liver fluke
(Fascioloides magna)

J 2016

Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ et. al.

Basic information

Original name

Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)

Authors

PRCHAL, Lukáš (203 Czech Republic), Ivan VOKŘÁL (203 Czech Republic), Martin KAŠNÝ (203 Czech Republic, guarantor, belonging to the institution), Lenka REJŠKOVÁ (203 Czech Republic), Markéta ZAJÍČKOVÁ (203 Czech Republic), Jiří LAMKA (203 Czech Republic), Lenka SKÁLOVÁ (203 Czech Republic), Lenka LECOVÁ (203 Czech Republic) and Barbora SZOTÁKOVÁ (203 Czech Republic)

Edition

Xenobiotica, UK, Taylor & Francis Group, 2016, 0049-8254

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.932

RIV identification code

RIV/00216224:14310/16:00093531

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3109/00498254.2015.1060370

UT WoS

000369275500005

Keywords in English

Albendazole; anthelmintics; biotransformation; drug-metabolizing enzymes; mebendazole

Abstract

V originále

1.Giant liver fluke Fascioloides magna is a dangerous parasite, which infects herbivores. It was imported to Europe from North America and started to spread. Benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. However their effect might be altered via drug-metabolizing enzymes of this parasite.2.The aim of our study was to determine the activities of drug-metabolizing enzymes in F. magna and the metabolism of above mentioned anthelmintics.3.Activities of several oxidative, reductive and conjugative enzymes towards various model xenobiotic substrates were found in F. magna subcellular fractions.4.Subcellular fractions from F. magna oxidized albendazole to its sulphoxide metabolite and reduced mebendazole to hydroxyl-mebendazole. Under ex vivo conditions, only very-low concentrations of these compounds were detected using high-performance liquid chromatography/mass spectrometry.5.The results indicate that the giant liver fluke possesses the active xenobiotic-metabolizing system. The overexpression of this system may play an important role in parasite resistance against these anthelmintics.

Links

MUNI/A/1325/2015, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)

Investor: Masaryk University, Category A

Citovat

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ and Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). Xenobiotica. UK: Taylor & Francis Group, 2016, vol. 46, No 2, p. 132-140. ISSN 0049-8254. Available from: https://dx.doi.org/10.3109/00498254.2015.1060370.

@article{1372489,
   author = {Prchal, Lukáš and Vokřál, Ivan and Kašný, Martin and Rejšková, Lenka and Zajíčková, Markéta and Lamka, Jiří and Skálová, Lenka and Lecová, Lenka and Szotáková, Barbora},
   article_location = {UK},
   article_number = {2},
   doi = {http://dx.doi.org/10.3109/00498254.2015.1060370},
   keywords = {Albendazole; anthelmintics; biotransformation; drug-metabolizing enzymes; mebendazole},
   language = {eng},
   issn = {0049-8254},
   journal = {Xenobiotica},
   title = {Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)},
   volume = {46},
   year = {2016}
}

TY  - JOUR
ID  - 1372489
AU  - Prchal, Lukáš - Vokřál, Ivan - Kašný, Martin - Rejšková, Lenka - Zajíčková, Markéta - Lamka, Jiří - Skálová, Lenka - Lecová, Lenka - Szotáková, Barbora
PY  - 2016
TI  - Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)
JF  - Xenobiotica
VL  - 46
IS  - 2
SP  - 132-140
EP  - 132-140
PB  - Taylor & Francis Group
SN  - 00498254
KW  - Albendazole
KW  - anthelmintics
KW  - biotransformation
KW  - drug-metabolizing enzymes
KW  - mebendazole
N2  - 1.Giant liver fluke Fascioloides magna is a dangerous parasite, which infects herbivores. It was imported to Europe from North America and started to spread. Benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. However their effect might be altered via drug-metabolizing enzymes of this parasite.2.The aim of our study was to determine the activities of drug-metabolizing enzymes in F. magna and the metabolism of above mentioned anthelmintics.3.Activities of several oxidative, reductive and conjugative enzymes towards various model xenobiotic substrates were found in F. magna subcellular fractions.4.Subcellular fractions from F. magna oxidized albendazole to its sulphoxide metabolite and reduced mebendazole to hydroxyl-mebendazole. Under ex vivo conditions, only very-low concentrations of these compounds were detected using high-performance liquid chromatography/mass spectrometry.5.The results indicate that the giant liver fluke possesses the active xenobiotic-metabolizing system. The overexpression of this system may play an important role in parasite resistance against these anthelmintics.
ER  -

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ and Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). \textit{Xenobiotica}. UK: Taylor \&{} Francis Group, 2016, vol.~46, No~2, p.~132-140. ISSN~0049-8254. Available from: https://dx.doi.org/10.3109/00498254.2015.1060370.

Detailed Information on Publication Record