Comparative Transcriptomic Exploration Reveals Unique Molecular
Adaptations of Neuropathogenic Trichobilharzia to Invade and
Parasitize Its Avian Definitive Host

J 2016

Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host

LEONTOVYČ, Roman, D Neil YOUNG, Pasi KORHONEN, S Hall HALL, Patrick TAN et. al.

Basic information

Original name

Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host

Authors

LEONTOVYČ, Roman (203 Czech Republic), D Neil YOUNG (36 Australia), Pasi KORHONEN (36 Australia), S Hall HALL (36 Australia), Patrick TAN (702 Singapore), Libor MIKEŠ (203 Czech Republic), Martin KAŠNÝ (203 Czech Republic, guarantor, belonging to the institution), Petr HORÁK (203 Czech Republic) and B Robin GASSER (36 Australia)

Edition

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2016, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.834

RIV identification code

RIV/00216224:14310/16:00093535

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pntd.0004406

UT WoS

000372567300026

Keywords in English

Trichobilharzia regenti; neuropathogen; transcriptome

Abstract

V originále

To date, most molecular investigations of schistosomatids have focused principally on blood flukes (schistosomes) of humans. Despite the clinical importance of cercarial dermatitis in humans caused by Trichobilharzia regenti and the serious neuropathologic disease that this parasite causes in its permissive avian hosts and accidental mammalian hosts, almost nothing is known about the molecular aspects of how this fluke invades its hosts, migrates in host tissues and how it interacts with its hosts' immune system. Here, we explored selected aspects using a transcriptomic-bioinformatic approach. To do this, we sequenced, assembled and annotated the transcriptome representing two consecutive life stages (cercariae and schistosomula) of T. regenti involved in the first phases of infection of the avian host. We identified key biological and metabolic pathways specific to each of these two developmental stages and also undertook comparative analyses using data available for taxonomically related blood flukes of the genus Schistosoma. Detailed comparative analyses revealed the unique involvement of carbohydrate metabolism, translation and amino acid metabolism, and calcium in T. regenti cercariae during their invasion and in growth and development, as well as the roles of cell adhesion molecules, microaerobic metabolism (citrate cycle and oxidative phosphorylation), peptidases (cathepsins) and other histolytic and lysozomal proteins in schistosomula during their particular migration in neural tissues of the avian host. In conclusion, the present transcriptomic exploration provides new and significant insights into the molecular biology of T. regenti, which should underpin future genomic and proteomic investigations of T. regenti and, importantly, provides a useful starting point for a range of comparative studies of schistosomatids and other trematodes.

Citovat

LEONTOVYČ, Roman, D Neil YOUNG, Pasi KORHONEN, S Hall HALL, Patrick TAN, Libor MIKEŠ, Martin KAŠNÝ, Petr HORÁK and B Robin GASSER. Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2016, vol. 10, No 2, p. "nestrankovano", 24 pp. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0004406.

@article{1372494,
   author = {Leontovyč, Roman and Young, D Neil and Korhonen, Pasi and Hall, S Hall and Tan, Patrick and Mikeš, Libor and Kašný, Martin and Horák, Petr and Gasser, B Robin},
   article_location = {San Francisco},
   article_number = {2},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0004406},
   keywords = {Trichobilharzia regenti; neuropathogen; transcriptome},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host},
   volume = {10},
   year = {2016}
}

TY  - JOUR
ID  - 1372494
AU  - Leontovyč, Roman - Young, D Neil - Korhonen, Pasi - Hall, S Hall - Tan, Patrick - Mikeš, Libor - Kašný, Martin - Horák, Petr - Gasser, B Robin
PY  - 2016
TI  - Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host
JF  - PLoS neglected tropical diseases
VL  - 10
IS  - 2
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Public Library of Science
SN  - 19352735
KW  - Trichobilharzia regenti
KW  - neuropathogen
KW  - transcriptome
N2  - To date, most molecular investigations of schistosomatids have focused principally on blood flukes (schistosomes) of humans. Despite the clinical importance of cercarial dermatitis in humans caused by Trichobilharzia regenti and the serious neuropathologic disease that this parasite causes in its permissive avian hosts and accidental mammalian hosts, almost nothing is known about the molecular aspects of how this fluke invades its hosts, migrates in host tissues and how it interacts with its hosts' immune system. Here, we explored selected aspects using a transcriptomic-bioinformatic approach. To do this, we sequenced, assembled and annotated the transcriptome representing two consecutive life stages (cercariae and schistosomula) of T. regenti involved in the first phases of infection of the avian host. We identified key biological and metabolic pathways specific to each of these two developmental stages and also undertook comparative analyses using data available for taxonomically related blood flukes of the genus Schistosoma. Detailed comparative analyses revealed the unique involvement of carbohydrate metabolism, translation and amino acid metabolism, and calcium in T. regenti cercariae during their invasion and in growth and development, as well as the roles of cell adhesion molecules, microaerobic metabolism (citrate cycle and oxidative phosphorylation), peptidases (cathepsins) and other histolytic and lysozomal proteins in schistosomula during their particular migration in neural tissues of the avian host. In conclusion, the present transcriptomic exploration provides new and significant insights into the molecular biology of T. regenti, which should underpin future genomic and proteomic investigations of T. regenti and, importantly, provides a useful starting point for a range of comparative studies of schistosomatids and other trematodes.
ER  -

LEONTOVYČ, Roman, D Neil YOUNG, Pasi KORHONEN, S Hall HALL, Patrick TAN, Libor MIKEŠ, Martin KAŠNÝ, Petr HORÁK and B Robin GASSER. Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2016, vol.~10, No~2, p.~''nestrankovano'', 24 pp. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0004406.

Detailed Information on Publication Record