Assessment of Chemical Impact of Invasive Bryozoan Pectinatella
magnifica on the Environment: Cytotoxicity and Antimicrobial
Activity of P. magnifica Extracts

J 2016

Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA et. al.

Basic information

Original name

Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts

Authors

KOLLAR, Peter (703 Slovakia), Karel SMEJKAL (203 Czech Republic), Hana SALMONOVA (203 Czech Republic), Eva VLKOVA (203 Czech Republic), Olga LEPSOVA-SKACELOVA (203 Czech Republic), Zuzana BALOUNOVA (203 Czech Republic), Josef RAJCHARD (203 Czech Republic), Josef CVACKA (203 Czech Republic), Libor JAŠA (203 Czech Republic, belonging to the institution), Pavel BABICA (203 Czech Republic, guarantor, belonging to the institution) and Jiří PAZOUREK (203 Czech Republic)

Edition

Molecules, BASEL, Mayer und Muller, 2016, 1420-3049

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10401 Organic chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.861

RIV identification code

RIV/00216224:14310/16:00093544

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/molecules21111476

UT WoS

000389918200058

Keywords in English

Aeromonas; antimicrobial activity; bacteria; Bryozoa; cyanobacteria; invasive species; Pectinatella magnifica; toxicity

Abstract

V originále

Pectinatella magnifica, an invasive bryozoan, might significantly affect ecosystem balance due to its massive occurrence in many areas in Europe and other parts of the world. Biological and chemical analyses are needed to get complete information about the impact of the animal on the environment. In this paper, we aimed to evaluate in vitro cytotoxic effects of five extracts prepared from P. magnifica using LDH assay on THP-1 cell line. Antimicrobial activities of extracts against 22 different bacterial strains were tested by microdilution method. Our study showed that all extracts tested, except aqueous portion, demonstrated LD50 values below 100 mu g/mL, which indicates potential toxicity. The water extract of P. magnifica with LD50 value of 250 mu g/mL also shows potentially harmful effects. Also, an environmental risk resulting from the presence and increasing biomass of potentially toxic benthic cyanobacteria in old colonies should not be underestimated. Toxicity of Pectinatella extracts could be partially caused by presence of Aeromonas species in material, since we found members of these genera as most abundant bacteria associated with P. magnifica. Furthermore, P. magnifica seems to be a promising source of certain antimicrobial agents. Its methanolic extract, hexane, and chloroform fractions possessed selective inhibitory effect on some potential pathogens and food spoiling bacteria in the range of MIC 0.5-10 mg/mL. Future effort should be made to isolate and characterize the content compounds derived from P. magnifica, which could help to identify the substance(s) responsible for the toxic effects of P. magnifica extracts.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA, Zuzana BALOUNOVA, Josef RAJCHARD, Josef CVACKA, Libor JAŠA, Pavel BABICA and Jiří PAZOUREK. Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts. Molecules. BASEL: Mayer und Muller, 2016, vol. 21, No 11, p. "nestrankovano", 14 pp. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules21111476.

@article{1372518,
   author = {Kollar, Peter and Smejkal, Karel and Salmonova, Hana and Vlkova, Eva and LepsovaandSkacelova, Olga and Balounova, Zuzana and Rajchard, Josef and Cvacka, Josef and Jaša, Libor and Babica, Pavel and Pazourek, Jiří},
   article_location = {BASEL},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/molecules21111476},
   keywords = {Aeromonas; antimicrobial activity; bacteria; Bryozoa; cyanobacteria; invasive species; Pectinatella magnifica; toxicity},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts},
   url = {http://www.mdpi.com/1420-3049/21/11/1476},
   volume = {21},
   year = {2016}
}

TY  - JOUR
ID  - 1372518
AU  - Kollar, Peter - Smejkal, Karel - Salmonova, Hana - Vlkova, Eva - Lepsova-Skacelova, Olga - Balounova, Zuzana - Rajchard, Josef - Cvacka, Josef - Jaša, Libor - Babica, Pavel - Pazourek, Jiří
PY  - 2016
TI  - Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts
JF  - Molecules
VL  - 21
IS  - 11
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Mayer und Muller
SN  - 14203049
KW  - Aeromonas
KW  - antimicrobial activity
KW  - bacteria
KW  - Bryozoa
KW  - cyanobacteria
KW  - invasive species
KW  - Pectinatella magnifica
KW  - toxicity
UR  - http://www.mdpi.com/1420-3049/21/11/1476
L2  - http://www.mdpi.com/1420-3049/21/11/1476
N2  - Pectinatella magnifica, an invasive bryozoan, might significantly affect ecosystem balance due to its massive occurrence in many areas in Europe and other parts of the world. Biological and chemical analyses are needed to get complete information about the impact of the animal on the environment. In this paper, we aimed to evaluate in vitro cytotoxic effects of five extracts prepared from P. magnifica using LDH assay on THP-1 cell line. Antimicrobial activities of extracts against 22 different bacterial strains were tested by microdilution method. Our study showed that all extracts tested, except aqueous portion, demonstrated LD50 values below 100 mu g/mL, which indicates potential toxicity. The water extract of P. magnifica with LD50 value of 250 mu g/mL also shows potentially harmful effects. Also, an environmental risk resulting from the presence and increasing biomass of potentially toxic benthic cyanobacteria in old colonies should not be underestimated. Toxicity of Pectinatella extracts could be partially caused by presence of Aeromonas species in material, since we found members of these genera as most abundant bacteria associated with P. magnifica. Furthermore, P. magnifica seems to be a promising source of certain antimicrobial agents. Its methanolic extract, hexane, and chloroform fractions possessed selective inhibitory effect on some potential pathogens and food spoiling bacteria in the range of MIC 0.5-10 mg/mL. Future effort should be made to isolate and characterize the content compounds derived from P. magnifica, which could help to identify the substance(s) responsible for the toxic effects of P. magnifica extracts.
ER  -

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA, Zuzana BALOUNOVA, Josef RAJCHARD, Josef CVACKA, Libor JAŠA, Pavel BABICA and Jiří PAZOUREK. Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts. \textit{Molecules}. BASEL: Mayer und Muller, 2016, vol.~21, No~11, p.~''nestrankovano'', 14 pp. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules21111476.

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