J 2016

Identification of metal sensitization in sarcoid-like metal-exposed patients by the MELISA (R) lymphocyte proliferation test - a pilot study

FIREMAN, Elizabeth, Amir BAR SHAI, Yifat ALCALAY, Noa OPHIR, Shmuel KIVITY et. al.

Basic information

Original name

Identification of metal sensitization in sarcoid-like metal-exposed patients by the MELISA (R) lymphocyte proliferation test - a pilot study

Authors

FIREMAN, Elizabeth (376 Israel), Amir BAR SHAI (376 Israel), Yifat ALCALAY (376 Israel), Noa OPHIR (376 Israel), Shmuel KIVITY (376 Israel) and Vera STEJSKAL (752 Sweden, guarantor, belonging to the institution)

Edition

JOURNAL OF OCCUPATIONAL MEDICINE AND TOXICOLOGY, LONDON, BIOMED CENTRAL LTD, 2016, 1745-6673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.482

RIV identification code

RIV/00216224:14310/16:00093557

Organization unit

Faculty of Science

DOI

UT WoS

000373769200002

Keywords in English

Granuloma; Inorganic exposure; Occupational diseases

Tags

Tags

International impact, Reviewed
Změněno: 6/4/2017 19:19, Ing. Andrea Mikešková

Abstract

V originále

Background: Pulmonary function is often affected by the inhalation of metal particles. The resulting pathology might trigger various lung diseases, e.g., parenchymal lung fibrosis and granulomatous lung disorders. We previously demonstrated that 6 % of tissue-proven sarcoid patients had a positive beryllium lymphocyte proliferation test (BeLPT), thus correcting the diagnosis to chronic beryllium disease. The aim of this study was to examine if MEmory Lymphocyte Immnuno Stimulation Assay (MELISA (R)), currently used for non-pulmonary diseases, can identify metals other than beryllium that can also trigger sensitization and induce granulomatous disease. Methods: This pilot study included 13 sarcoid-like patients who underwent MELISA (R). Eleven patients also underwent BeLPT. Biopsy samples were tested for metal content by scanning electron microscope. Eleven study patients had been exposed to metals at the workplace and 2 had silicone implants. Results: Two patients who had undergone BeLPT were positive for beryllium. MELISA (R) detected 9 patients (9/13, 69 %) who were positive for at least one of the tested metals: 4 reacted positively to nickel, 4 to titanium, 2 to chromium, 2 to beryllium, 2 to silica, and one each to palladium, mercury and lead. Conclusion: It is proposed that MELISA (R) can be exploited to also identify specific sensitization in individuals exposed to inhaled particles from a variety of metals.