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@article{1372607,
author = {Norton, Pamela and Comunale, Mary Ann and Herrera, Harmin and Wang, Mengjun and Houser, Josef and Wimmerová, Michaela and Romano, Patrick R and Mehta, Anand},
article_location = {HOBOKEN},
article_number = {24},
doi = {http://dx.doi.org/10.1002/pmic.201600064},
keywords = {Aleuria aurantia lectin; Biomarker; Glycoproteomics; Glycosylation; Hepatocellular carcinoma; Liver cancer},
language = {eng},
issn = {1615-9853},
journal = {Proteomics},
title = {Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma},
url = {http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract},
volume = {16},
year = {2016}
}
TY - JOUR
ID - 1372607
AU - Norton, Pamela - Comunale, Mary Ann - Herrera, Harmin - Wang, Mengjun - Houser, Josef - Wimmerová, Michaela - Romano, Patrick R - Mehta, Anand
PY - 2016
TI - Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma
JF - Proteomics
VL - 16
IS - 24
SP - 3126-3136
EP - 3126-3136
PB - Wiley-Blackwell
SN - 16159853
KW - Aleuria aurantia lectin
KW - Biomarker
KW - Glycoproteomics
KW - Glycosylation
KW - Hepatocellular carcinoma
KW - Liver cancer
UR - http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract
N2 - The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in -1,2, -1,3, -1,4, and -1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.
ER -
NORTON, Pamela, Mary Ann COMUNALE, Harmin HERRERA, Mengjun WANG, Josef HOUSER, Michaela WIMMEROVÁ, Patrick R ROMANO and Anand MEHTA. Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma. \textit{Proteomics}. HOBOKEN: Wiley-Blackwell, 2016, vol.~16, No~24, p.~3126-3136. ISSN~1615-9853. Available from: https://dx.doi.org/10.1002/pmic.201600064.
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