MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Analysis of the acute response of Galleria mellonella larvae to potassium nitrate. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology. New York, USA: ELSEVIER SCIENCE INC, vol. 195, May, p. 44-51. ISSN 1532-0456. 2017.
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Basic information
Original name Analysis of the acute response of Galleria mellonella larvae to potassium nitrate
Authors MAGUIRE, Ronan (372 Ireland), Martin KUNC (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution) and Kevin KAVANAGH (372 Ireland).
Edition Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, New York, USA, ELSEVIER SCIENCE INC, 2017, 1532-0456.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30102 Immunology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.426
RIV identification code RIV/00216224:14310/17:00096165
Organization unit Faculty of Science
UT WoS 000398875100005
Keywords (in Czech) Galleria mellonella; In vivo; Potassium nitrate; Toxicity;
Keywords in English Galleria mellonella; In vivo; Potassium nitrate; Toxicity;
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 28/3/2018 14:57.
Abstract
Potassium nitrate (E252) is widely used as a food preservative and has applications in the treatment of high blood pressure however high doses are carcinogenic. Larvae of Galleria mellonella were adminstered potassium nitrate to establish whether the acute effects in larvae correlated with those evident in mammals. Intra-haemocoel injection of potassium nitrate resulted in a significant increase in the densisty of circulating haemocytes and a small change in the relative proportions of haemocytes but haemocytes showed a reduced fungicidal ability. Potassium nitrate administration resulted in increased superoxide dimutase activity and in the abundance of a range of proteins associated with mitochondrial function (e.g. mitochondrial aldehyde dehydrogenase, putative mitochondrial Mn superoxide dismutase), metabolism (e.g. triosephosphate isomerase, glyceraldehye 3 phosphate dehydrogenase) and nitrate metabolism (e.g. aliphatic nitrilase, glutathione S-transferase). A strong correlation exists between the toxicity of a range of food preservatives when tested in G. mellonella larvae and rats. In this work a correlation between the effect of potassium nitrate in larvae and mammals is shown and opens the way to the utilisation of insects for studying the in vivo acute and chronic toxicity of xenobiotics.
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