MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Analysis of the acute response of Galleria mellonella larvae to potassium nitrate. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology. New York, USA: ELSEVIER SCIENCE INC, vol. 195, May, p. 44-51. ISSN 1532-0456. 2017. |
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@article{1372627, author = {Maguire, Ronan and Kunc, Martin and Hyršl, Pavel and Kavanagh, Kevin}, article_location = {New York, USA}, article_number = {May}, keywords = {Galleria mellonella; In vivo; Potassium nitrate; Toxicity;}, language = {eng}, issn = {1532-0456}, journal = {Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology}, title = {Analysis of the acute response of Galleria mellonella larvae to potassium nitrate}, volume = {195}, year = {2017} }
TY - JOUR ID - 1372627 AU - Maguire, Ronan - Kunc, Martin - Hyršl, Pavel - Kavanagh, Kevin PY - 2017 TI - Analysis of the acute response of Galleria mellonella larvae to potassium nitrate JF - Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology VL - 195 IS - May SP - 44-51 EP - 44-51 PB - ELSEVIER SCIENCE INC SN - 15320456 KW - Galleria mellonella KW - In vivo KW - Potassium nitrate KW - Toxicity; N2 - Potassium nitrate (E252) is widely used as a food preservative and has applications in the treatment of high blood pressure however high doses are carcinogenic. Larvae of Galleria mellonella were adminstered potassium nitrate to establish whether the acute effects in larvae correlated with those evident in mammals. Intra-haemocoel injection of potassium nitrate resulted in a significant increase in the densisty of circulating haemocytes and a small change in the relative proportions of haemocytes but haemocytes showed a reduced fungicidal ability. Potassium nitrate administration resulted in increased superoxide dimutase activity and in the abundance of a range of proteins associated with mitochondrial function (e.g. mitochondrial aldehyde dehydrogenase, putative mitochondrial Mn superoxide dismutase), metabolism (e.g. triosephosphate isomerase, glyceraldehye 3 phosphate dehydrogenase) and nitrate metabolism (e.g. aliphatic nitrilase, glutathione S-transferase). A strong correlation exists between the toxicity of a range of food preservatives when tested in G. mellonella larvae and rats. In this work a correlation between the effect of potassium nitrate in larvae and mammals is shown and opens the way to the utilisation of insects for studying the in vivo acute and chronic toxicity of xenobiotics. ER -
MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Analysis of the acute response of Galleria mellonella larvae to potassium nitrate. \textit{Comparative Biochemistry and Physiology - Part C: Toxicology \&{} Pharmacology}. New York, USA: ELSEVIER SCIENCE INC, vol.~195, May, p.~44-51. ISSN~1532-0456. 2017.
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