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@inbook{1372749,
author = {Babica, Pavel and Sovadinová, Iva and Upham, Brad L.},
address = {New York, USA},
booktitle = {Gap Junction Protocols},
doi = {http://dx.doi.org/10.1007/978-1-4939-3664-9},
editor = {Vinken, Mathieu; Johnstone, Scott R.},
keywords = {Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers},
howpublished = {elektronická verze "online"},
language = {eng},
location = {New York, USA},
isbn = {978-1-4939-3664-9},
pages = {133-144},
publisher = {Springer New York},
title = {Scrape Loading/Dye Transfer Assay},
url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9},
year = {2016}
}
TY - CHAP
ID - 1372749
AU - Babica, Pavel - Sovadinová, Iva - Upham, Brad L.
PY - 2016
TI - Scrape Loading/Dye Transfer Assay
VL - Methods in Molecular Biology
PB - Springer New York
CY - New York, USA
SN - 9781493936649
KW - Dye coupling
KW - Dye transfer
KW - Ex vivo assessment
KW - Gap junctional intercellular communication assessment
KW - High throughput
KW - In vitro assay
KW - Incision loading
KW - Lucifer Yellow
KW - Scalpel loading
KW - Scrape loading
KW - Tracers
UR - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
L2 - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
N2 - The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
ER -
BABICA, Pavel, Iva SOVADINOVÁ a Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. In Vinken, Mathieu; Johnstone, Scott R. \textit{Gap Junction Protocols}. New York, USA: Springer New York. s.~133-144. Methods in Molecular Biology. ISBN~978-1-4939-3664-9. doi:10.1007/978-1-4939-3664-9. 2016.
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