Scrape Loading/Dye Transfer Assay

C 2016

Scrape Loading/Dye Transfer Assay

BABICA, Pavel, Iva SOVADINOVÁ and Brad L. UPHAM

Basic information

Original name

Scrape Loading/Dye Transfer Assay

Authors

BABICA, Pavel (203 Czech Republic, guarantor, belonging to the institution), Iva SOVADINOVÁ (203 Czech Republic, belonging to the institution) and Brad L. UPHAM (840 United States of America)

Edition

New York, USA, Gap Junction Protocols, p. 133-144, 12 pp. Methods in Molecular Biology, 2016

Publisher

Springer New York

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

References:

URL

RIV identification code

RIV/00216224:14310/16:00093583

Organization unit

Faculty of Science

ISBN

978-1-4939-3664-9

DOI

http://dx.doi.org/10.1007/978-1-4939-3664-9

UT WoS

000381766400010

Keywords in English

Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers

Abstract

V originále

The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.

Links

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BABICA, Pavel, Iva SOVADINOVÁ and Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. Gap Junction Protocols. New York, USA: Springer New York, 2016, p. 133-144. Methods in Molecular Biology. ISBN 978-1-4939-3664-9. Available from: https://dx.doi.org/10.1007/978-1-4939-3664-9.

@inbook{1372749,
   author = {Babica, Pavel and Sovadinová, Iva and Upham, Brad L.},
   address = {New York, USA},
   booktitle = {Gap Junction Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-3664-9},
   editor = {Vinken, Mathieu; Johnstone, Scott R.},
   keywords = {Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-4939-3664-9},
   pages = {133-144},
   publisher = {Springer New York},
   title = {Scrape Loading/Dye Transfer Assay},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9},
   year = {2016}
}

TY  - CHAP
ID  - 1372749
AU  - Babica, Pavel - Sovadinová, Iva - Upham, Brad L.
PY  - 2016
TI  - Scrape Loading/Dye Transfer Assay
VL  - Methods in Molecular Biology
PB  - Springer New York
CY  - New York, USA
SN  - 9781493936649
KW  - Dye coupling
KW  - Dye transfer
KW  - Ex vivo assessment
KW  - Gap junctional intercellular communication assessment
KW  - High throughput
KW  - In vitro assay
KW  - Incision loading
KW  - Lucifer Yellow
KW  - Scalpel loading
KW  - Scrape loading
KW  - Tracers
UR  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
L2  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
N2  - The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
ER  -

BABICA, Pavel, Iva SOVADINOVÁ and Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. \textit{Gap Junction Protocols}. New York, USA: Springer New York, 2016, p.~133-144. Methods in Molecular Biology. ISBN~978-1-4939-3664-9. Available from: https://dx.doi.org/10.1007/978-1-4939-3664-9.

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