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@article{1372843,
author = {Oralová, Veronika and Matalová, Eva and Killinger, Michael and Knopfová, Lucia and Šmarda, Jan and Buchtová, marcela},
article_location = {New York},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s00223-016-0219-2},
keywords = {mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array},
language = {eng},
issn = {0171-967X},
journal = {Calcified Tissue International},
title = {Osteogenic potential of the transcription factor c-MYB},
volume = {100},
year = {2017}
}
TY - JOUR
ID - 1372843
AU - Oralová, Veronika - Matalová, Eva - Killinger, Michael - Knopfová, Lucia - Šmarda, Jan - Buchtová, marcela
PY - 2017
TI - Osteogenic potential of the transcription factor c-MYB
JF - Calcified Tissue International
VL - 100
IS - 3
SP - 311-322
EP - 311-322
PB - Springer
SN - 0171967X
KW - mineralised matrix
KW - micromass cultures
KW - mouse limbs
KW - osteogenesis
KW - PCR Array
N2 - Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
ER -
ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA and marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. \textit{Calcified Tissue International}. New York: Springer, 2017, vol.~100, No~3, p.~311-322. ISSN~0171-967X. Available from: https://dx.doi.org/10.1007/s00223-016-0219-2.
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