Detailed Information on Publication Record
2017
Osteogenic potential of the transcription factor c-MYB
ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA et. al.Basic information
Original name
Osteogenic potential of the transcription factor c-MYB
Authors
ORALOVÁ, Veronika (203 Czech Republic), Eva MATALOVÁ (203 Czech Republic), Michael KILLINGER (203 Czech Republic, belonging to the institution), Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and marcela BUCHTOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Calcified Tissue International, New York, Springer, 2017, 0171-967X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.293
RIV identification code
RIV/00216224:14310/17:00094639
Organization unit
Faculty of Science
UT WoS
000395129400011
Keywords in English
mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array
Změněno: 10/4/2018 13:01, Ing. Nicole Zrilić
Abstract
V originále
Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
Links
| GB14-37368G, research and development project |
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