Osteogenic potential of the transcription factor c-MYB

J 2017

Osteogenic potential of the transcription factor c-MYB

ORALOVÁ, Veronika; Eva MATALOVÁ; Michael KILLINGER; Lucia KNOPFOVÁ; Jan ŠMARDA et. al.

Basic information

Original name

Osteogenic potential of the transcription factor c-MYB

Authors

ORALOVÁ, Veronika (203 Czech Republic); Eva MATALOVÁ (203 Czech Republic); Michael KILLINGER (203 Czech Republic, belonging to the institution); Lucia KNOPFOVÁ (203 Czech Republic, belonging to the institution); Jan ŠMARDA (203 Czech Republic, belonging to the institution) and marcela BUCHTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Calcified Tissue International, New York, Springer, 2017, 0171-967X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.293

RIV identification code

RIV/00216224:14310/17:00094639

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00223-016-0219-2

UT WoS

000395129400011

EID Scopus

2-s2.0-85007242104

Keywords in English

mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array

Abstract

V originále

Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.

Links

GB14-37368G, research and development project

Name: Centrum orofaciálního vývoje a regenerace

Investor: Czech Science Foundation

Cite

ORALOVÁ, Veronika; Eva MATALOVÁ; Michael KILLINGER; Lucia KNOPFOVÁ; Jan ŠMARDA and marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. Calcified Tissue International. New York: Springer, 2017, vol. 100, No 3, p. 311-322. ISSN 0171-967X. Available from: https://dx.doi.org/10.1007/s00223-016-0219-2.

@article{1372843,
   author = {Oralová, Veronika and Matalová, Eva and Killinger, Michael and Knopfová, Lucia and Šmarda, Jan and Buchtová, marcela},
   article_location = {New York},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00223-016-0219-2},
   keywords = {mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array},
   language = {eng},
   issn = {0171-967X},
   journal = {Calcified Tissue International},
   title = {Osteogenic potential of the transcription factor c-MYB},
   volume = {100},
   year = {2017}
}

TY  - JOUR
ID  - 1372843
AU  - Oralová, Veronika - Matalová, Eva - Killinger, Michael - Knopfová, Lucia - Šmarda, Jan - Buchtová, marcela
PY  - 2017
TI  - Osteogenic potential of the transcription factor c-MYB
JF  - Calcified Tissue International
VL  - 100
IS  - 3
SP  - 311-322
EP  - 311-322
PB  - Springer
SN  - 0171967X
KW  - mineralised matrix
KW  - micromass cultures
KW  - mouse limbs
KW  - osteogenesis
KW  - PCR Array
N2  - Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
ER  -

ORALOVÁ, Veronika; Eva MATALOVÁ; Michael KILLINGER; Lucia KNOPFOVÁ; Jan ŠMARDA and marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. \textit{Calcified Tissue International}. New York: Springer, 2017, vol.~100, No~3, p.~311-322. ISSN~0171-967X. Available from: https://dx.doi.org/10.1007/s00223-016-0219-2.

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