Evaluation of the infectivity and the persistence of
Trichinella patagoniensis in muscle tissue of decomposing
guinea pig (Cavia porcellus)

J 2017

Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)

FARINA, Fernando, Marianna PASQUALETTI, Jana ILGOVÁ, Natalia CARDILLO, Mariano ERCOLE et. al.

Basic information

Original name

Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)

Authors

FARINA, Fernando (32 Argentina), Marianna PASQUALETTI (32 Argentina), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Natalia CARDILLO (32 Argentina), Mariano ERCOLE (32 Argentina), Tatiana ARONOWICZ (32 Argentina), Silvio KRIVOKAPICH (32 Argentina), Martin KAŠNÝ (203 Czech Republic, guarantor, belonging to the institution) and Mabel RIBICICH (32 Argentina)

Edition

Parasitology Research, NEW YORK, Springer, 2017, 0932-0113

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.558

RIV identification code

RIV/00216224:14310/17:00096174

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00436-016-5299-4

UT WoS

000390568500038

Keywords (in Czech)

Trichinella patagoniensis; Přežívání v rozkládající se tkáni; Infektivita; Guinea pigs; Cavia porcellus

Keywords in English

Trichinella patagoniensis; Persistence in decomposing muscle tissue; Infectivity; Guinea pigs; Cavia porcellus

Abstract

V originále

Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014-2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 degrees C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.

Links

MUNI/A/1325/2015, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)

Investor: Masaryk University, Category A

Citovat

FARINA, Fernando, Marianna PASQUALETTI, Jana ILGOVÁ, Natalia CARDILLO, Mariano ERCOLE, Tatiana ARONOWICZ, Silvio KRIVOKAPICH, Martin KAŠNÝ and Mabel RIBICICH. Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus). Parasitology Research. NEW YORK: Springer, 2017, vol. 116, No 1, p. 371-375. ISSN 0932-0113. Available from: https://dx.doi.org/10.1007/s00436-016-5299-4.

@article{1373037,
   author = {Farina, Fernando and Pasqualetti, Marianna and Ilgová, Jana and Cardillo, Natalia and Ercole, Mariano and Aronowicz, Tatiana and Krivokapich, Silvio and Kašný, Martin and Ribicich, Mabel},
   article_location = {NEW YORK},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s00436-016-5299-4},
   keywords = {Trichinella patagoniensis; Persistence in decomposing muscle tissue; Infectivity; Guinea pigs; Cavia porcellus},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)},
   url = {https://link.springer.com/article/10.1007%2Fs00436-016-5299-4},
   volume = {116},
   year = {2017}
}

TY  - JOUR
ID  - 1373037
AU  - Farina, Fernando - Pasqualetti, Marianna - Ilgová, Jana - Cardillo, Natalia - Ercole, Mariano - Aronowicz, Tatiana - Krivokapich, Silvio - Kašný, Martin - Ribicich, Mabel
PY  - 2017
TI  - Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus)
JF  - Parasitology Research
VL  - 116
IS  - 1
SP  - 371-375
EP  - 371-375
PB  - Springer
SN  - 09320113
KW  - Trichinella patagoniensis
KW  - Persistence in decomposing muscle tissue
KW  - Infectivity
KW  - Guinea pigs
KW  - Cavia porcellus
UR  - https://link.springer.com/article/10.1007%2Fs00436-016-5299-4
L2  - https://link.springer.com/article/10.1007%2Fs00436-016-5299-4
N2  - Trichinella patagoniensis, a new species of Trichinella, is widespread in Argentina. The success of parasite transmission depends, among other factors, on the resistance of L1 larvae present in the muscle tissue (ML) of dead hosts undergoing the decomposition process in different environmental conditions. The aim of the present work was to study the infectivity of T. patagoniensis muscle larvae in Cavia porcellus and the capability of the parasite to survive in decomposed muscle tissue of guinea pigs subjected to different environmental conditions. Thirty-two female Ssi:AL guinea pigs were orally inoculated with 2000 ML of T. patagoniensis (ISS2311). All the animals were sacrificed 42 days post-infection. Twenty-six animals were eviscerated, and carcasses were placed on the surface of soil inside plastic boxes that were exposed to environmental conditions in the summer 2014-2015 and autumn of 2015 in Buenos Aires, Argentina. Carcasses from six animals were placed into a plastic box inside the refrigerator at a temperature of 4 degrees C. The muscle tissue samples from the carcasses were examined weekly for the presence of larvae, and the infectivity of recovered ML was tested in BALB/c mice. Our results showed for the first time the ability of T. patagoniensis to complete its life cycle in guinea pigs, thus serving as a potential natural host. Also, larvae of T. patagoniensis remained infective in muscle tissue for several weeks while undergoing decomposition under different environmental conditions.
ER  -

FARINA, Fernando, Marianna PASQUALETTI, Jana ILGOVÁ, Natalia CARDILLO, Mariano ERCOLE, Tatiana ARONOWICZ, Silvio KRIVOKAPICH, Martin KAŠNÝ and Mabel RIBICICH. Evaluation of the infectivity and the persistence of Trichinella patagoniensis in muscle tissue of decomposing guinea pig (Cavia porcellus). \textit{Parasitology Research}. NEW YORK: Springer, 2017, vol.~116, No~1, p.~371-375. ISSN~0932-0113. Available from: https://dx.doi.org/10.1007/s00436-016-5299-4.

Detailed Information on Publication Record