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@article{1373251,
author = {Pavlištová, Veronika and Dvořáčková, Martina and Jež, Michal and Mozgová, Iva and Mokroš, Petr and Fajkus, Jiří},
article_location = {Hoboken},
article_number = {3},
doi = {http://dx.doi.org/10.1111/tpj.13257},
keywords = {CHROMATIN ASSEMBLY FACTOR-1; RIBOSOMAL-RNA GENES; DOSAGE CONTROL; FACTOR CAF-1; REPEATS; TRANSFORMATION; RECOMBINATION; MAINTENANCE; DYSFUNCTION; SITES},
language = {eng},
issn = {0960-7412},
journal = {Plant Journal},
title = {Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes},
url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract},
volume = {88},
year = {2016}
}
TY - JOUR
ID - 1373251
AU - Pavlištová, Veronika - Dvořáčková, Martina - Jež, Michal - Mozgová, Iva - Mokroš, Petr - Fajkus, Jiří
PY - 2016
TI - Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes
JF - Plant Journal
VL - 88
IS - 3
SP - 411-424
EP - 411-424
PB - Wiley-Blackwell
SN - 09607412
KW - CHROMATIN ASSEMBLY FACTOR-1
KW - RIBOSOMAL-RNA GENES
KW - DOSAGE CONTROL
KW - FACTOR CAF-1
KW - REPEATS
KW - TRANSFORMATION
KW - RECOMBINATION
KW - MAINTENANCE
KW - DYSFUNCTION
KW - SITES
UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract
N2 - Arabidopsis thaliana mutants dysfunctional in the evolutionarily conserved protein complex chromatin assembly factor-1 (CAF-1), which deposits the canonical histone H3 variant H3.1 during DNA synthesis-dependent chromatin assembly, display complex phenotypic changes including meristem and growth alterations, sensitivity to DNA-damaging agents, and reduced fertility. We reported previously that mutants in the FAS1 subunit of CAF-1 progressively lose telomere and 45S rDNA repeats. Here we show that multiple aspects of the fas phenotype are recovered immediately on expression of a reintroduced FAS1 allele, and are clearly independent of the recovery of rDNA copy-numbers and telomeres. In reverted lines, 45S rDNA genes are recovered to diverse levels with a strikingly different representation of their variants, and the typical association of nucleolar organizing region 4 with the nucleolus is perturbed. One of 45S rDNA variants (VAR1), which is silenced in wild-type (WT) plants without mutation history (Col-0 WT), dominates the expression pattern, whereas VAR2 is dominant in Col-0 WT plants. We propose an explanation for the variability of telomere and 45S rDNA repeats associated with CAF-1 function, suggesting that the differences in nuclear partitioning and expression of the rDNA variants in fas mutants and their revertants provide a useful experimental system to study genetic and epigenetic factors in gene dosage compensation.
ER -
PAVLIŠTOVÁ, Veronika, Martina DVOŘÁČKOVÁ, Michal JEŽ, Iva MOZGOVÁ, Petr MOKROŠ and Jiří FAJKUS. Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2016, vol.~88, No~3, p.~411-424. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.13257.
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