J 2016

Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes

PAVLIŠTOVÁ, Veronika, Martina DVOŘÁČKOVÁ, Michal JEŽ, Iva MOZGOVÁ, Petr MOKROŠ et. al.

Basic information

Original name

Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes

Authors

PAVLIŠTOVÁ, Veronika (203 Czech Republic, belonging to the institution), Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), Michal JEŽ (203 Czech Republic, belonging to the institution), Iva MOZGOVÁ (203 Czech Republic, belonging to the institution), Petr MOKROŠ (203 Czech Republic, belonging to the institution) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Hoboken, Wiley-Blackwell, 2016, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.901

RIV identification code

RIV/00216224:14740/16:00088685

Organization unit

Central European Institute of Technology

DOI

UT WoS

000389202800006

Keywords in English

CHROMATIN ASSEMBLY FACTOR-1; RIBOSOMAL-RNA GENES; DOSAGE CONTROL; FACTOR CAF-1; REPEATS; TRANSFORMATION; RECOMBINATION; MAINTENANCE; DYSFUNCTION; SITES

Tags

Změněno: 14/3/2017 13:39, Mgr. Eva Špillingová

Abstract

V originále

Arabidopsis thaliana mutants dysfunctional in the evolutionarily conserved protein complex chromatin assembly factor-1 (CAF-1), which deposits the canonical histone H3 variant H3.1 during DNA synthesis-dependent chromatin assembly, display complex phenotypic changes including meristem and growth alterations, sensitivity to DNA-damaging agents, and reduced fertility. We reported previously that mutants in the FAS1 subunit of CAF-1 progressively lose telomere and 45S rDNA repeats. Here we show that multiple aspects of the fas phenotype are recovered immediately on expression of a reintroduced FAS1 allele, and are clearly independent of the recovery of rDNA copy-numbers and telomeres. In reverted lines, 45S rDNA genes are recovered to diverse levels with a strikingly different representation of their variants, and the typical association of nucleolar organizing region 4 with the nucleolus is perturbed. One of 45S rDNA variants (VAR1), which is silenced in wild-type (WT) plants without mutation history (Col-0 WT), dominates the expression pattern, whereas VAR2 is dominant in Col-0 WT plants. We propose an explanation for the variability of telomere and 45S rDNA repeats associated with CAF-1 function, suggesting that the differences in nuclear partitioning and expression of the rDNA variants in fas mutants and their revertants provide a useful experimental system to study genetic and epigenetic factors in gene dosage compensation.

Links

GAP501/11/0289, research and development project
Name: Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin
Investor: Czech Science Foundation
GA16-01137S, research and development project
Name: Faktory genomové stability u mechu a vyšších rostlin
Investor: Czech Science Foundation
GJ16-04166Y, research and development project
Name: Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis
Investor: Czech Science Foundation
LH15189, research and development project
Name: Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR