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@article{1373363,
author = {Danhel, Ales and Trosanova, Zuzana and Balintova, Jana and Havran, Ludek and Hocek, Michal and Barek, Jiri and Fojta, Miroslav},
article_location = {OXFORD},
article_number = {October},
doi = {http://dx.doi.org/10.1016/j.electacta.2016.08.096},
keywords = {Aromatic Azide; Enzymatic Incorporation; Mercury Electrode; Nucleic Acid; Redox Labeling; Voltammetry},
language = {eng},
issn = {0013-4686},
journal = {Electrochimica Acta},
title = {Voltammetric analysis of 5-(4-Azidophenyl)-2 '-deoxycytidine nucleoside and azidophenyl-labelled single- and double-stranded DNAs},
url = {http://www.sciencedirect.com/science/article/pii/S0013468616318205},
volume = {215},
year = {2016}
}
TY - JOUR
ID - 1373363
AU - Danhel, Ales - Trosanova, Zuzana - Balintova, Jana - Havran, Ludek - Hocek, Michal - Barek, Jiri - Fojta, Miroslav
PY - 2016
TI - Voltammetric analysis of 5-(4-Azidophenyl)-2 '-deoxycytidine nucleoside and azidophenyl-labelled single- and double-stranded DNAs
JF - Electrochimica Acta
VL - 215
IS - October
SP - 72-83
EP - 72-83
PB - Pergamon-Elsevier
SN - 00134686
KW - Aromatic Azide
KW - Enzymatic Incorporation
KW - Mercury Electrode
KW - Nucleic Acid
KW - Redox Labeling
KW - Voltammetry
UR - http://www.sciencedirect.com/science/article/pii/S0013468616318205
L2 - http://www.sciencedirect.com/science/article/pii/S0013468616318205
N2 - Voltammetric determination of a redox labeled nucleoside 5-(4-azidophenyl)-2'-deoxycytidine (dC(AZP)) and various polymerase-synthesized dC(AZP)-labeled DNAs in aqueous buffers is presented. Influence of: i) pH (2-12), ii) scan rates (0.02-10 V s(-1)), and iii) dC(AZP) concentration (0.02-10 mu mol l(-1)), on voltammograms of dC(AZP) were systematically studied for the first time using CV at a hanging mercury drop electrode. Electrode potential-controlled adsorption driven process allowed sensitive determination of dC(AZP) at nanomolar concentrations using adsorptive stripping voltammetry. Transfer stripping voltammetry (TSV) was used for the detection of dC(AZP)-labeled DNA in femtomole quantities. Precise sequence-specific incorporation of dC(AZP) into DNA by primer extension was used to demonstrate a perfect correlation between the number of incorporated AZP moieties and TSV responses. In addition, for the first time we used polymerase chain reaction to prepare an about 350-bp double-stranded DNA fragment globally modified with dC(AZP), and of terminal deoxynucleotidyl transferase tailing reaction to generate end-labeled single stranded oligonucleotides. Effects of DNA structure on the AZP-modified DNA TSV responses are discussed. (C) 2016 Elsevier Ltd. All rights reserved.
ER -
DANHEL, Ales, Zuzana TROSANOVA, Jana BALINTOVA, Ludek HAVRAN, Michal HOCEK, Jiri BAREK a Miroslav FOJTA. Voltammetric analysis of 5-(4-Azidophenyl)-2 '-deoxycytidine nucleoside and azidophenyl-labelled single- and double-stranded DNAs. \textit{Electrochimica Acta}. OXFORD: Pergamon-Elsevier, roč.~215, October, s.~72-83. ISSN~0013-4686. doi:10.1016/j.electacta.2016.08.096. 2016.
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