Detailed Information on Publication Record
2016
High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium
ŠVEC, Pavel, Petr PETRÁŠ, Roman PANTŮČEK, Jiří DOŠKAŘ, Ivo SEDLÁČEK et. al.Basic information
Original name
High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)
Edition
International Journal of Systematic and Evolutionary Microbiology, Society for General Microbiology, Great Britain, 2016, 1466-5026
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.134
RIV identification code
RIV/00216224:14310/16:00093669
Organization unit
Faculty of Science
UT WoS
000393357900046
Keywords in English
Staphylococcus sciuri; subspecies; reclassification; taxonomy
Tags
International impact, Reviewed
Změněno: 26/10/2020 10:43, prof. RNDr. Roman Pantůček, Ph.D.
Abstract
V originále
A polyphasic taxonomic approach was applied to strains of the species Staphylococcus sciuri in order to clarify the taxonomic legitimacy of the delineation of S. sciuri into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. A group of 81 S. sciuri isolates obtained from human (n=62) and veterinary (n=17) clinical materials and foods (n=2) and ten reference and type strains obtained from the Czech Collection of Microorganisms were characterized by extensive biotyping using conventional tests and commercial identification kits (ID 32 Staph, STAPHYtest, Biolog Microbial ID System), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, automated ribotyping with EcoRI restriction enzyme, 16S–23S rRNA gene intergenic transcribed spacer PCR fingerprinting and repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Selected strains representing different ribotypes were further characterized using sequencing of the beta-subunit of RNA polymerase (rpoB) gene. Individual techniques revealed high heterogeneity within the analysed S. sciuri strains but differentiation of the investigated strains into groups corresponding to the aforementioned S. sciuri subspecies and supported by these techniques was not clearly revealed. Based on obtained results and data retrieved from literature we propose rejecting the separation of S. sciuri species into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium and we suggest reclassification these subspecies as S. sciuri with the type strain W.E. Kloos SC 116T (=ATCC 29062T=BCRC 12927T=CCM 3473T=CCUG 15598T=CNCTC 5683T=DSM 20345T=JCM 2425T=NCTC 12103T).
Links
| MUNI/A/0967/2015, interní kód MU |
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