Detailed Information on Publication Record
2016
Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny
DLUHOŠOVÁ, Jana, Jana ŘEPKOVÁ, Hana JAKEŠOVÁ and Jan NEDĚLNÍKBasic information
Original name
Impact of Interspecific Hybridization of T. pratense x T. medium and Backcrossing on Genetic Variability of Progeny
Authors
DLUHOŠOVÁ, Jana (203 Czech Republic, belonging to the institution), Jana ŘEPKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Hana JAKEŠOVÁ (203 Czech Republic) and Jan NEDĚLNÍK (203 Czech Republic)
Edition
Czech Journal of Genetics and Plant Breeding, Praha, Czech Academy of Agricultural Sciences, 2016, 1212-1975
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10603 Genetics and heredity
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.532
RIV identification code
RIV/00216224:14310/16:00087683
Organization unit
Faculty of Science
UT WoS
000390388200001
Keywords in English
cytology; FISH; hybrids; rDNA; red clover; zigzag clover
Tags
Tags
International impact, Reviewed
Změněno: 14/3/2018 10:10, prof. RNDr. Jana Řepková, CSc.
Abstract
V originále
Red clover (Trifolium pratense L.) is a high-quality fodder crop which has been hybridized successfully with its wild relative zigzag clover (T. medium L.). The aim of this study was to evaluate the genetic impact of interspecific hybridization and subsequent repeated backcrossing on the variability within hybrid progeny genomes. Nuclear DNA content of 800 and 753 hybrid plants from F-7/F-8 and F-8/F-9 generations, respectively, was measured by flow cytometry. Resulting values were converted to estimated chromosome counts, which were successfully validated on a sample of 28 plants by counting mitotic chromosomes. The two generations showed a similar distribution of various chromosome counts ranging from 22 to 47 chromosomes. In total, 24.0% and 34.3% of plants from the two generations had different numbers of chromosomes from their parental plants. Variability within the hybrid population was assessed by fluorescent in situ hybridization using rDNA probes. Individual plants had a pattern of 5S and 45S rDNA loci rather more similar to that of T. pratense than of T. medium. Numbers of chromosomes with clusters of 5S rDNA ranged from 6 to 14 while those of 45S rDNA varied between 4 and 13. Individual arrangements were almost unique, and some plants possessed also novel formations which were not present in any of the parental species, such as a cluster of 5S rDNA surrounded by 45S rDNA clusters or a 45S rDNA cluster surrounded by 5S rDNA clusters. This suggests complex rearrangements connected with post-hybridization stabilization of hybrid genomes.
Links
| QI111A019, research and development project |
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