BOGDANOVIČOVÁ, Kateřina, Lenka NECIDOVÁ, Danka HARUŠTIAKOVÁ and Bohumíra JANŠTOVÁ. Milk powder risk assessment with staphylococcus aureus toxigenic strains. In Petros Taoukis, George John Nychas. 29th International Conference of the European-Federation-of-Food-Science-and-Technology (EFFoST). Oxford: Elsevier Science Ltd. p. 2-7. ISSN 0956-7135. doi:10.1016/j.foodcont.2016.07.007. 2017.
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Basic information
Original name Milk powder risk assessment with staphylococcus aureus toxigenic strains
Authors BOGDANOVIČOVÁ, Kateřina (203 Czech Republic), Lenka NECIDOVÁ (203 Czech Republic), Danka HARUŠTIAKOVÁ (703 Slovakia, guarantor, belonging to the institution) and Bohumíra JANŠTOVÁ (203 Czech Republic).
Edition Oxford, 29th International Conference of the European-Federation-of-Food-Science-and-Technology (EFFoST), p. 2-7, 6 pp. 2017.
Publisher Elsevier Science Ltd
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 30308 Nutrition, Dietetics
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
Impact factor Impact factor: 3.667
RIV identification code RIV/00216224:14110/17:00096205
Organization unit Faculty of Medicine
ISSN 0956-7135
Doi http://dx.doi.org/10.1016/j.foodcont.2016.07.007
UT WoS 000390636300002
Keywords in English Staphylococcal enterotoxins; Powdered milk; ELFA
Tags EL OK, podil
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 17/5/2018 15:57.
Abstract
Staphylococcus aureus is the causative agent of staphylococcal enterotoxicosis foodborne illnesS. Milk powder and whey powder are at risk of contamination by coagulase-positive staphylococci, which reflects the requirement for microbiological examination of foods listed in Regulation (EC) 2073/2005, as amended. Microbiological criteria for coagulase-positive staphylococci are up to 10(1)-10(2) cfu g(-1). This study evaluates the possibility of survival and growth of S. aureus in milk powder after its reconstitution. Powdered milk was inoculated with 10(2) and 10(5) cfu g(-1) of toxigenic strains of S. aureus and then stored as reconstituted milk for 48 h at 4,15, and 25 degrees C. Staphylococcal growth and production of staphylococcal enterotoxins A, B, and C (SEA, SEB, and SEC) was regularly detected during the 48 h storage period. With inoculation of S. aureus high counts at 25 degrees C, the production of staphylococcal enterotoxins (SEs) was detected as early as after 7 (SEB) or 8 (SEA) hours of storage; at 15 degrees C as early as after 48 h (SEA, SEB). With inoculation of low counts of S. aureus (complying with legislative requirements) only at 25 degrees C, SEs production was detected after 24 (SEA, SEB) or 48 (SEA, SEB, SEC) hours. Model experiments evaluated SEs consumer risk resulting from extended storage of reconstituted milk at improper temperatures. (C) 2016 Elsevier Ltd. All rights reserved.
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