Cryo-EM study of slow bee paralysis virus at low pH reveals
iflavirus genome release mechanism

J 2017

Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

KALYNYCH, Sergei, Tibor FÜZIK, Antonin PRIDAL, Joachim DE MIRANDA, Pavel PLEVKA et. al.

Basic information

Original name

Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism

Authors

KALYNYCH, Sergei (124 Canada, belonging to the institution), Tibor FÜZIK (703 Slovakia, belonging to the institution), Antonin PRIDAL (203 Czech Republic), Joachim DE MIRANDA (752 Sweden) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2017, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.504

RIV identification code

RIV/00216224:14740/17:00096214

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1073/pnas.1616562114

UT WoS

000392095800054

Keywords in English

electron microscopy; uncoating; honeybee; structure; virus

Abstract

V originále

Viruses from the family Iflaviridae are insect pathogens. Many of them, including slow bee paralysis virus (SBPV), cause lethal diseases in honeybees and bumblebees, resulting in agricultural losses. Iflaviruses have nonenveloped icosahedral virions containing single-stranded RNA genomes. However, their genome release mechanism is unknown. Here, we show that low pH promotes SBPV genome release, indicating that the virus may use endosomes to enter host cells. We used cryo-EM to study a heterogeneous population of SBPV virions at pH 5.5. We determined the structures of SBPV particles before and after genome release to resolutions of 3.3 and 3.4 angstrom, respectively. The capsids of SBPV virions in low pH are not expanded. Thus, SBPV does not appear to form "altered" particles with pores in their capsids before genome release, as is the case in many related picornaviruses. The egress of the genome from SBPV virions is associated with a loss of interpentamer contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the capsid. Pores that are 7 angstrom an diameter form around icosahedral threefold symmetry axes. We speculate that they serve as channels for the genome release. Our findings provide an atomic-level characterization of the genome release mechanism of iflaviruses.

Links

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

KALYNYCH, Sergei, Tibor FÜZIK, Antonin PRIDAL, Joachim DE MIRANDA and Pavel PLEVKA. Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2017, vol. 114, No 3, p. 598-603. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1616562114.

@article{1373930,
   author = {Kalynych, Sergei and Füzik, Tibor and Pridal, Antonin and de Miranda, Joachim and Plevka, Pavel},
   article_location = {WASHINGTON},
   article_number = {3},
   doi = {http://dx.doi.org/10.1073/pnas.1616562114},
   keywords = {electron microscopy; uncoating; honeybee; structure; virus},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism},
   url = {http://www.pnas.org/content/114/3/598},
   volume = {114},
   year = {2017}
}

TY  - JOUR
ID  - 1373930
AU  - Kalynych, Sergei - Füzik, Tibor - Pridal, Antonin - de Miranda, Joachim - Plevka, Pavel
PY  - 2017
TI  - Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 114
IS  - 3
SP  - 598-603
EP  - 598-603
PB  - National Academy of Sciences
SN  - 00278424
KW  - electron microscopy
KW  - uncoating
KW  - honeybee
KW  - structure
KW  - virus
UR  - http://www.pnas.org/content/114/3/598
L2  - http://www.pnas.org/content/114/3/598
N2  - Viruses from the family Iflaviridae are insect pathogens. Many of them, including slow bee paralysis virus (SBPV), cause lethal diseases in honeybees and bumblebees, resulting in agricultural losses. Iflaviruses have nonenveloped icosahedral virions containing single-stranded RNA genomes. However, their genome release mechanism is unknown. Here, we show that low pH promotes SBPV genome release, indicating that the virus may use endosomes to enter host cells. We used cryo-EM to study a heterogeneous population of SBPV virions at pH 5.5. We determined the structures of SBPV particles before and after genome release to resolutions of 3.3 and 3.4 angstrom, respectively. The capsids of SBPV virions in low pH are not expanded. Thus, SBPV does not appear to form "altered" particles with pores in their capsids before genome release, as is the case in many related picornaviruses. The egress of the genome from SBPV virions is associated with a loss of interpentamer contacts mediated by N-terminal arms of VP2 capsid proteins, which result in the expansion of the capsid. Pores that are 7 angstrom an diameter form around icosahedral threefold symmetry axes. We speculate that they serve as channels for the genome release. Our findings provide an atomic-level characterization of the genome release mechanism of iflaviruses.
ER  -

KALYNYCH, Sergei, Tibor FÜZIK, Antonin PRIDAL, Joachim DE MIRANDA and Pavel PLEVKA. Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2017, vol.~114, No~3, p.~598-603. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1616562114.

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