Virion Structure of Israeli Acute Bee Paralysis Virus

MULLAPUDI, Edukondalu, Antonin PRIDAL, Lenka PÁLKOVÁ, Joachim R. DE MIRANDA and Pavel PLEVKA. Virion Structure of Israeli Acute Bee Paralysis Virus. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2016, vol. 90, No 18, p. 8150-8159. ISSN 0022-538X. Available from: https://dx.doi.org/10.1128/JVI.00854-16.

Basic information

• Original name: Virion Structure of Israeli Acute Bee Paralysis Virus
• Authors: MULLAPUDI, Edukondalu (356 India, belonging to the institution), Antonin PRIDAL (203 Czech Republic), Lenka PÁLKOVÁ (203 Czech Republic, belonging to the institution), Joachim R. DE MIRANDA (752 Sweden) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2016, 0022-538X.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.663
• RIV identification code: RIV/00216224:14740/16:00093719
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1128/JVI.00854-16
• UT WoS: 000382314100011
• Keywords in English: COLONY COLLAPSE DISORDER; 3-DIMENSIONAL STRUCTURE; POLIOVIRUS; RESOLUTION; PARTICLES; GENOME; LOSSES; CRYSTALLOGRAPHY; PICORNAVIRUSES; IDENTIFICATION
• Tags: rivok

Abstract

The pollination services provided by the western honeybee (Apis mellifera) are critical for agricultural production and the diversity of wild flowering plants. However, honeybees suffer from environmental pollution, habitat loss, and pathogens, including viruses that can cause fatal diseases. Israeli acute bee paralysis virus (IAPV), from the family Dicistroviridae, has been shown to cause colony collapse disorder in the United States. Here, we present the IAPV virion structure determined to a resolution of 4.0 angstrom and and the structure of a pentamer of capsid protein protomers at a resolution of 2.7 angstrom. IAPV has major capsid proteins VP1 and VP3 with noncanonical jellyroll beta-barrel folds composed of only seven instead of eight beta-strands, as is the rule for proteins of other viruses with the same fold. The maturation of dicistroviruses is connected to the cleavage of precursor capsid protein VP0 into subunits VP3 and VP4. We show that a putative catalytic site formed by the residues Asp-Asp-Phe of VP1 is optimally positioned to perform the cleavage. Furthermore, unlike many picornaviruses, IAPV does not contain a hydrophobic pocket in capsid protein VP1 that could be targeted by capsid-binding antiviral compounds. IMPORTANCE Honeybee pollination is required for agricultural production and to sustain the biodiversity of wild flora. However, honeybee populations in Europe and North America are under pressure from pathogens, including viruses that cause colony losses. Viruses from the family Dicistroviridae can cause honeybee infections that are lethal, not only to individual honeybees, but to whole colonies. Here, we present the virion structure of an Aparavirus, Israeli acute bee paralysis virus (IAPV), a member of a complex of closely related viruses that are distributed worldwide. IAPV exhibits unique structural features not observed in other picorna-like viruses. Capsid protein VP1 of IAPV does not contain a hydrophobic pocket, implying that capsid-binding antiviral compounds that can prevent the replication of vertebrate picornaviruses may be ineffective against honeybee virus infections.