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@article{1373982,
author = {Watson, J. Matthew and Platzer, Alexander and Kazda, Anita and Akimcheva, Svetlana and Valuchová, Soňa and Nizhynska, Viktoria and Nordborg, Magnus and Říha, Karel},
article_location = {WASHINGTON},
article_number = {43},
doi = {http://dx.doi.org/10.1073/pnas.1609686113},
keywords = {mutation rate; shoot apical meristem; germline; mismatch repair; telomeres},
language = {eng},
issn = {0027-8424},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
title = {Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis},
url = {http://www.pnas.org/content/113/43/12226},
volume = {113},
year = {2016}
}
TY - JOUR
ID - 1373982
AU - Watson, J. Matthew - Platzer, Alexander - Kazda, Anita - Akimcheva, Svetlana - Valuchová, Soňa - Nizhynska, Viktoria - Nordborg, Magnus - Říha, Karel
PY - 2016
TI - Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis
JF - Proceedings of the National Academy of Sciences of the United States of America
VL - 113
IS - 43
SP - 12226-12231
EP - 12226-12231
PB - National Academy of Sciences
SN - 00278424
KW - mutation rate
KW - shoot apical meristem
KW - germline
KW - mismatch repair
KW - telomeres
UR - http://www.pnas.org/content/113/43/12226
L2 - http://www.pnas.org/content/113/43/12226
N2 - In plants, gametogenesis occurs late in development, and somatic mutations can therefore be transmitted to the next generation. Longer periods of growth are believed to result in an increase in the number of cell divisions before gametogenesis, with a concomitant increase in mutations arising due to replication errors. However, there is little experimental evidence addressing how many cell divisions occur before gametogenesis. Here, we measured loss of telomeric DNA and accumulation of replication errors in Arabidopsis with short and long life spans to determine the number of replications in lineages leading to gametes. Surprisingly, the number of cell divisions within the gamete lineage is nearly independent of both life span and vegetative growth. One consequence of the relatively stable number of replications per generation is that older plants may not pass along more somatically acquired mutations to their offspring. We confirmed this hypothesis by genomic sequencing of progeny from young and old plants. This independence can be achieved by hierarchical arrangement of cell divisions in plant meristems where vegetative growth is primarily accomplished by expansion of cells in rapidly dividing meristematic zones, which are only rarely refreshed by occasional divisions of more quiescent cells. We support this model by 5-ethynyl-2'-deoxyuridine retention experiments in shoot and root apical meristems. These results suggest that stem-cell organization has independently evolved in plants and animals to minimize mutations by limiting DNA replication.
ER -
WATSON, J. Matthew, Alexander PLATZER, Anita KAZDA, Svetlana AKIMCHEVA, Soňa VALUCHOVÁ, Viktoria NIZHYNSKA, Magnus NORDBORG and Karel ŘÍHA. Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2016, vol.~113, No~43, p.~12226-12231. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1609686113.
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