PIN6 auxin transporter at endoplasmic reticulum and plasma
membrane mediates auxin homeostasis and organogenesis in
Arabidopsis

J 2016

PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

SIMON, Sibu, Petr SKUPA, Tom VIAENE, Marta ZWIEWKA, Ricardo TEJOS et. al.

Basic information

Original name

PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

Authors

SIMON, Sibu (356 India, belonging to the institution), Petr SKUPA (203 Czech Republic), Tom VIAENE (56 Belgium), Marta ZWIEWKA (616 Poland, guarantor, belonging to the institution), Ricardo TEJOS (56 Belgium), Petr KLIMA (203 Czech Republic), Maria CARNA (203 Czech Republic), Jakub ROLCIK (203 Czech Republic), Riet DE RYCKE (56 Belgium), Ignacio MORENO (152 Chile), Petre I. DOBREV (203 Czech Republic), Ariel ORELLANA (152 Chile), Eva ZAZIMALOVA (203 Czech Republic) and Jiri FRIML (203 Czech Republic)

Edition

New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2016, 0028-646X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.330

RIV identification code

RIV/00216224:14740/16:00088715

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/nph.14019

UT WoS

000379212800008

Keywords in English

auxin; endoplasmic reticulum (ER); lateral root; PIN; plasma membrane (PM)

Abstract

V originále

Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

Citovat

SIMON, Sibu, Petr SKUPA, Tom VIAENE, Marta ZWIEWKA, Ricardo TEJOS, Petr KLIMA, Maria CARNA, Jakub ROLCIK, Riet DE RYCKE, Ignacio MORENO, Petre I. DOBREV, Ariel ORELLANA, Eva ZAZIMALOVA and Jiri FRIML. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist. HOBOKEN: WILEY-BLACKWELL, 2016, vol. 211, No 1, p. 65-74. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.14019.

@article{1373985,
   author = {Simon, Sibu and Skupa, Petr and Viaene, Tom and Zwiewka, Marta and Tejos, Ricardo and Klima, Petr and Carna, Maria and Rolcik, Jakub and De Rycke, Riet and Moreno, Ignacio and Dobrev, Petre I. and Orellana, Ariel and Zazimalova, Eva and Friml, Jiri},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/nph.14019},
   keywords = {auxin; endoplasmic reticulum (ER); lateral root; PIN; plasma membrane (PM)},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.14019/abstract;jsessionid=10FCD031F6E3F17A23746DD5AD2F3EF2.f04t04},
   volume = {211},
   year = {2016}
}

TY  - JOUR
ID  - 1373985
AU  - Simon, Sibu - Skupa, Petr - Viaene, Tom - Zwiewka, Marta - Tejos, Ricardo - Klima, Petr - Carna, Maria - Rolcik, Jakub - De Rycke, Riet - Moreno, Ignacio - Dobrev, Petre I. - Orellana, Ariel - Zazimalova, Eva - Friml, Jiri
PY  - 2016
TI  - PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis
JF  - New Phytologist
VL  - 211
IS  - 1
SP  - 65-74
EP  - 65-74
PB  - WILEY-BLACKWELL
SN  - 0028646X
KW  - auxin
KW  - endoplasmic reticulum (ER)
KW  - lateral root
KW  - PIN
KW  - plasma membrane (PM)
UR  - http://onlinelibrary.wiley.com/doi/10.1111/nph.14019/abstract;jsessionid=10FCD031F6E3F17A23746DD5AD2F3EF2.f04t04
L2  - http://onlinelibrary.wiley.com/doi/10.1111/nph.14019/abstract;jsessionid=10FCD031F6E3F17A23746DD5AD2F3EF2.f04t04
N2  - Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
ER  -

SIMON, Sibu, Petr SKUPA, Tom VIAENE, Marta ZWIEWKA, Ricardo TEJOS, Petr KLIMA, Maria CARNA, Jakub ROLCIK, Riet DE RYCKE, Ignacio MORENO, Petre I. DOBREV, Ariel ORELLANA, Eva ZAZIMALOVA and Jiri FRIML. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. \textit{New Phytologist}. HOBOKEN: WILEY-BLACKWELL, 2016, vol.~211, No~1, p.~65-74. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.14019.

Detailed Information on Publication Record