MAREČEK, Vít, Alexandr MIKYŠKA, David HAMPEL, Pavel ČEJKA, Jana NEUWIRTHOVÁ, Alexandra MALACHOVÁ and Radim CERKAL. ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt. Journal of Cereal Science. London: Academic Press Inc., 2017, vol. 73, January 2017, p. 40-45. ISSN 0733-5210. Available from: https://dx.doi.org/10.1016/j.jcs.2016.11.004.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt
Name (in English) ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt
Authors MAREČEK, Vít (203 Czech Republic), Alexandr MIKYŠKA (203 Czech Republic), David HAMPEL (203 Czech Republic), Pavel ČEJKA (203 Czech Republic), Jana NEUWIRTHOVÁ (203 Czech Republic, guarantor, belonging to the institution), Alexandra MALACHOVÁ (203 Czech Republic) and Radim CERKAL (203 Czech Republic).
Edition Journal of Cereal Science, London, Academic Press Inc. 2017, 0733-5210.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 40101 Agriculture
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.302
RIV identification code RIV/00216224:14110/17:00096220
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.jcs.2016.11.004
UT WoS 000395224800007
Keywords in English Barley; Malting; Trolox; Antioxidant capacity determination
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 21/3/2018 13:27.
Abstract
Many analytical methods for antioxidant determination in foodstuffs and raw materials based on various principles have been published so far. However, not all of them are applicable to barley and malt. The results of total antioxidant capacity (TEAC) of barley and malt obtained with methods based on ability to eliminate radicals of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) were compared and statistically evaluated. The average TEAC of barley determined using ABTS and DPPH ranged from 2.1 to 2.5 micromol g-1 and from 1.2 to 1.7 micromol g-1, respectively. The TEAC ranges in malt were 2.7-3.0 micromol g-1 (ABTS) and 1.8-2.6 micromol g-1 (DPPH). TEAC of barley and malt were affected by the weather conditions (year), variety and application of Zn2+ fertilizer. The ABTS and the DPPH methods represent an effective tool for the assessment of antioxidant capacity of spring barley and malt and the factors having an impact on it.
Abstract (in English)
Many analytical methods for antioxidant determination in foodstuffs and raw materials based on various principles have been published so far. However, not all of them are applicable to barley and malt. The results of total antioxidant capacity (TEAC) of barley and malt obtained with methods based on ability to eliminate radicals of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) were compared and statistically evaluated. The average TEAC of barley determined using ABTS and DPPH ranged from 2.1 to 2.5 micromol g-1 and from 1.2 to 1.7 micromol g-1, respectively. The TEAC ranges in malt were 2.7-3.0 micromol g-1 (ABTS) and 1.8-2.6 micromol g-1 (DPPH). TEAC of barley and malt were affected by the weather conditions (year), variety and application of Zn2+ fertilizer. The ABTS and the DPPH methods represent an effective tool for the assessment of antioxidant capacity of spring barley and malt and the factors having an impact on it.
PrintDisplayed: 8/5/2024 04:04