Painting of Arabidopsis Chromosomes with Chromosome-Specific
BAC Clones

J 2016

Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

MANDÁKOVÁ, Terezie and Martin LYSÁK

Basic information

Original name

Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

Authors

MANDÁKOVÁ, Terezie (203 Czech Republic, belonging to the institution) and Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Current protocols in plant biology, John Wiley & Sons, 2016, 2379-8068

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/16:00093748

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/cppb.20022

Keywords in English

BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes

Abstract

V originále

Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MANDÁKOVÁ, Terezie and Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 4, No 2, p. 359-371. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20022.

@article{1374157,
   author = {Mandáková, Terezie and Lysák, Martin},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/cppb.20022},
   keywords = {BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes},
   language = {eng},
   issn = {2379-8068},
   journal = {Current protocols in plant biology},
   title = {Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract},
   volume = {4},
   year = {2016}
}

TY  - JOUR
ID  - 1374157
AU  - Mandáková, Terezie - Lysák, Martin
PY  - 2016
TI  - Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones
JF  - Current protocols in plant biology
VL  - 4
IS  - 2
SP  - 359-371
EP  - 359-371
PB  - John Wiley & Sons
SN  - 23798068
KW  - BAC FISH
KW  - Brassicaceae
KW  - chromosome painting
KW  - fluorescence in situ hybridization (FISH)
KW  - nick translation
KW  - pachytene chromosomes
UR  - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
N2  - Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.
ER  -

MANDÁKOVÁ, Terezie and Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. \textit{Current protocols in plant biology}. John Wiley \&{} Sons, 2016, vol.~4, No~2, p.~359-371. ISSN~2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20022.

Detailed Information on Publication Record