J 2016

Chromosome Preparation for Cytogenetic Analyses in Arabidopsis

MANDÁKOVÁ, Terezie and Martin LYSÁK

Basic information

Original name

Chromosome Preparation for Cytogenetic Analyses in Arabidopsis

Authors

MANDÁKOVÁ, Terezie (203 Czech Republic, belonging to the institution) and Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Current protocols in plant biology, John Wiley & Sons, 2016, 2379-8068

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14740/16:00088725

Organization unit

Central European Institute of Technology

DOI

Keywords in English

arabidopsis; Brassicaceae; mitotic and meiotic chromosomes; pachytene

Tags

Změněno: 28/2/2017 15:23, Mgr. Eva Špillingová

Abstract

V originále

Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step-by-step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana. Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae).

Links

GBP501/12/G090, research and development project
Name: Evoluce a funkce komplexních genomů rostlin