| MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. American Journal of Botany. St Louis: Botanical Soc Amer Inc, 2016, roč. 103, č. 7, s. 1187-1196. ISSN 0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452. |
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@article{1374270,
author = {Mandáková, Terezie and Gloss, Andrew D. and Whiteman, Noah K. and Lysák, Martin},
article_location = {St Louis},
article_number = {7},
doi = {http://dx.doi.org/10.3732/ajb.1500452},
keywords = {Brassicaceae; centromere loss; chromosome fusion; chromosome translocation; diploidization; dysploidy; karyotype evolution; polyploidy; whole-genome duplication},
language = {eng},
issn = {0002-9122},
journal = {American Journal of Botany},
title = {How diploidization turned a tetraploid into a pseudotriploid},
url = {http://www.amjbot.org/content/103/7/1187},
volume = {103},
year = {2016}
}
TY - JOUR
ID - 1374270
AU - Mandáková, Terezie - Gloss, Andrew D. - Whiteman, Noah K. - Lysák, Martin
PY - 2016
TI - How diploidization turned a tetraploid into a pseudotriploid
JF - American Journal of Botany
VL - 103
IS - 7
SP - 1187-1196
EP - 1187-1196
PB - Botanical Soc Amer Inc
SN - 00029122
KW - Brassicaceae
KW - centromere loss
KW - chromosome fusion
KW - chromosome translocation
KW - diploidization
KW - dysploidy
KW - karyotype evolution
KW - polyploidy
KW - whole-genome duplication
UR - http://www.amjbot.org/content/103/7/1187
L2 - http://www.amjbot.org/content/103/7/1187
N2 - PREMISE OF THE STUDY: Despite being highly fertile and occupying a large geographic region, the North American heartleaf bittercress (Cardamine cordifolia; Brassicaceae) has a puzzling triploid- like chromosome number (2n = 3x = 24). As most triploids are sterile, we embarked on a detailed analysis of the C. cordifolia genome to elucidate its origin and structure. METHODS: Mitotic and meiotic chromosome complement of C. cordifolia was analyzed by comparative chromosome painting using chromosome-specific BAC contigs of Arabidopsis thaliana. Resulting chromosome patterns were documented by multicolor fluorescence microscopy and compared with known ancestral and extant Brassicaceae genomes. KEY RESULTS: We discovered that C. cordifolia is not a triploid hybrid but a diploidized tetraploid with the prevalence of regular, diploid-like meiotic pairing. The ancestral tetraploid chromosome number (2n = 32) was reduced to a triploid- like number (2n = 24) through four terminal chromosome translocations. CONCLUSIONS: The structure of the pseudotriploid C. cordifolia genome results from a stepwise diploidization process after whole-genome duplication. We showed that translocation-based descending dysploidy (from n = 16 to n = 12) was mediated by the formation of five new chromosomes. The genome of C. cordifolia represents the diploidization process in statu nascendi and provides valuable insights into mechanisms of postpolyploidy rediploidization in land plants. Our data further suggest that chromosome number alone does not need to be a reliable proxy of species' evolutionary past and that the same chromosome number may originate either by polyploidization (hybridization) or due to descending dysploidy.
ER -
MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. \textit{American Journal of Botany}. St Louis: Botanical Soc Amer Inc, 2016, roč.~103, č.~7, s.~1187-1196. ISSN~0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452.
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