Utilization of LA-ICP-MS for bioimaging

k 2016

Utilization of LA-ICP-MS for bioimaging

VACULOVIČ, Tomáš, Michaela TVRDOŇOVÁ, Michal MASAŘÍK, Hana POLANSKÁ, Markéta SVOBODOVÁ et. al.

Basic information

Original name

Utilization of LA-ICP-MS for bioimaging

Authors

VACULOVIČ, Tomáš (203 Czech Republic, guarantor, belonging to the institution), Michaela TVRDOŇOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Hana POLANSKÁ (203 Czech Republic, belonging to the institution), Markéta SVOBODOVÁ (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Zuzana ČADKOVÁ (203 Czech Republic), Vítězslav OTRUBA (203 Czech Republic, belonging to the institution) and Jiřina SZÁKOVÁ (203 Czech Republic)

Edition

European Symposium on Atomic Spectrometry, 2016

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10406 Analytical chemistry

Country of publisher

Hungary

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/16:00088752

Organization unit

Faculty of Science

Keywords in English

Trends and new techniques of atomic spectrometry; Atomic absorption; atomic fluorescence; atomic emission methods; X-ray spectrometry; Mass spectrometry;

Abstract

V originále

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) represents one of the most suitable method for study of lateral distribution of specific elements (Cu, Zn, Pb, Pt...) in low concentration levels (tens mg/kg) in various biological tissues. Presented work is focused on study of influence of scan speed and laser spot diameter on lateral resolution, LOD, and time consumption of analysis. The utilization of LA-ICP-MS for bioimaging purpose will be demonstrated on two different types of the matrices. First one is tumour tissue which is treated by various Pt-based cytostatics (carbo-, oxali- and cis-Pt). The second one are cuts of tapeworm treated by various heavy metals.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-18154S, research and development project

Name: Prvkové mapování těžkých kovů v rostlinných a živočišných akumulátorech; kde se akumulují?

Investor: Czech Science Foundation

Citovat

VACULOVIČ, Tomáš, Michaela TVRDOŇOVÁ, Michal MASAŘÍK, Hana POLANSKÁ, Markéta SVOBODOVÁ, Jaromír GUMULEC, Viktor KANICKÝ, Zuzana ČADKOVÁ, Vítězslav OTRUBA and Jiřina SZÁKOVÁ. Utilization of LA-ICP-MS for bioimaging. In European Symposium on Atomic Spectrometry. 2016.

@proceedings{1374973,
   author = {Vaculovič, Tomáš and Tvrdoňová, Michaela and Masařík, Michal and Polanská, Hana and Svobodová, Markéta and Gumulec, Jaromír and Kanický, Viktor and Čadková, Zuzana and Otruba, Vítězslav and Száková, Jiřina},
   booktitle = {European Symposium on Atomic Spectrometry},
   keywords = {Trends and new techniques of atomic spectrometry; Atomic absorption; atomic fluorescence; atomic emission methods; X-ray spectrometry; Mass spectrometry;},
   language = {eng},
   title = {Utilization of LA-ICP-MS for bioimaging},
   year = {2016}
}

TY  - CONF
ID  - 1374973
AU  - Vaculovič, Tomáš - Tvrdoňová, Michaela - Masařík, Michal - Polanská, Hana - Svobodová, Markéta - Gumulec, Jaromír - Kanický, Viktor - Čadková, Zuzana - Otruba, Vítězslav - Száková, Jiřina
PY  - 2016
TI  - Utilization of LA-ICP-MS for bioimaging
KW  - Trends and new techniques of atomic spectrometry
KW  - Atomic absorption
KW  - atomic fluorescence
KW  - atomic emission methods
KW  - X-ray spectrometry
KW  - Mass spectrometry;
N2  - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) represents one of the most suitable method for study of lateral distribution of specific elements (Cu, Zn, Pb, Pt...) in low concentration levels (tens mg/kg) in various biological tissues. Presented work is focused on study of influence of scan speed and laser spot diameter on lateral resolution, LOD, and time consumption of analysis. The utilization of LA-ICP-MS for bioimaging purpose will be demonstrated on two different types of the matrices. First one is tumour tissue which is treated by various Pt-based cytostatics (carbo-, oxali- and cis-Pt). The second one are cuts of tapeworm treated by various heavy metals.
ER  -

Detailed Information on Publication Record