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@article{1374999,
author = {Kolářová, Lenka and Prokeš, Lubomír and Kučera, Lukáš and Hampl, Aleš and Peňa Mendéz, Eladia Maria and Vaňhara, Petr and Havel, Josef},
article_location = {New York},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s13361-016-1567-x},
keywords = {Monoisotopic elements; Calibration; Gold clusters; Phosphorus clusters; TOF mass spectrometry},
language = {eng},
issn = {1044-0305},
journal = {Journal of The American Society for Mass Spectrometry},
title = {Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry},
url = {https://link.springer.com/article/10.1007%2Fs13361-016-1567-x},
volume = {28},
year = {2017}
}
TY - JOUR
ID - 1374999
AU - Kolářová, Lenka - Prokeš, Lubomír - Kučera, Lukáš - Hampl, Aleš - Peňa Mendéz, Eladia Maria - Vaňhara, Petr - Havel, Josef
PY - 2017
TI - Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry
JF - Journal of The American Society for Mass Spectrometry
VL - 28
IS - 3
SP - 419-427
EP - 419-427
PB - Springer
SN - 10440305
KW - Monoisotopic elements
KW - Calibration
KW - Gold clusters
KW - Phosphorus clusters
KW - TOF mass spectrometry
UR - https://link.springer.com/article/10.1007%2Fs13361-016-1567-x
N2 - Precise calibration in TOF MS requires suitable and reliable standards, which are not always available for high masses. We evaluated inorganic clusters of the monoisotopic elements gold and phosphorus (Aun+/Aun- and Pn+/Pn-) as an alternative to peptides or proteins for the external and internal calibration of mass spectra in various experimental and instrumental scenarios. Monoisotopic gold or phosphorus clusters can be easily generated in situ from suitable precursors by laser desorption/ionization (LDI) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Their use offers numerous advantages, including simplicity of preparation, biological inertness, and exact mass determination even at lower mass resolution. We used citrate-stabilized gold nanoparticles to generate gold calibration clusters, and red phosphorus powder to generate phosphorus clusters. Both elements can be added to samples to perform internal calibration up to mass-to-charge (m/z) 10–15,000 without significantly interfering with the analyte. We demonstrated the use of the gold and phosphorous clusters in the MS analysis of complex biological samples, including microbial standards and total extracts of mouse embryonic fibroblasts. We believe that clusters of monoisotopic elements could be used as generally applicable calibrants for complex biological samples.
ER -
KOLÁŘOVÁ, Lenka, Lubomír PROKEŠ, Lukáš KUČERA, Aleš HAMPL, Eladia Maria PEŇA MENDÉZ, Petr VAŇHARA a Josef HAVEL. Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry. \textit{Journal of The American Society for Mass Spectrometry}. New York: Springer, 2017, roč.~28, č.~3, s.~419-427. ISSN~1044-0305. Dostupné z: https://dx.doi.org/10.1007/s13361-016-1567-x.
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