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@article{1375030,
author = {Strbkova, L. and Manakhov, Anton and Zajíčková, Lenka and Stoica, Adrian and Vesely, P. and Chmelik, R.},
article_location = {LAUSANNE},
article_number = {June},
doi = {http://dx.doi.org/10.1016/j.surfcoat.2015.10.076},
keywords = {Plasma polymer; Cyclopropylamine; Cell adhesion; Holographic microscopy; Quantitative phase imaging},
language = {eng},
issn = {0257-8972},
journal = {SURFACE & COATINGS TECHNOLOGY},
title = {The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy},
url = {http://www.sciencedirect.com/science/article/pii/S0257897215303728},
volume = {295},
year = {2016}
}
TY - JOUR
ID - 1375030
AU - Strbkova, L. - Manakhov, Anton - Zajíčková, Lenka - Stoica, Adrian - Vesely, P. - Chmelik, R.
PY - 2016
TI - The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy
JF - SURFACE & COATINGS TECHNOLOGY
VL - 295
IS - June
SP - 70-77
EP - 70-77
PB - ELSEVIER SCIENCE SA
SN - 02578972
KW - Plasma polymer
KW - Cyclopropylamine
KW - Cell adhesion
KW - Holographic microscopy
KW - Quantitative phase imaging
UR - http://www.sciencedirect.com/science/article/pii/S0257897215303728
L2 - http://www.sciencedirect.com/science/article/pii/S0257897215303728
N2 - The understanding of cell-surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell-surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasma treated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation. (C) 2015 The Authors. Published by Elsevier B.V.
ER -
STRBKOVA, L., Anton MANAKHOV, Lenka ZAJÍČKOVÁ, Adrian STOICA, P. VESELY and R. CHMELIK. The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy. \textit{SURFACE \&{} COATINGS TECHNOLOGY}. LAUSANNE: ELSEVIER SCIENCE SA, 2016, vol.~295, June, p.~70-77. ISSN~0257-8972. Available from: https://dx.doi.org/10.1016/j.surfcoat.2015.10.076.
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