The robust bio-immobilization based on pulsed plasma
polymerization of cyclopropylamine and glutaraldehyde coupling
chemistry

J 2016

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

MANAKHOV, Anton, Ekaterina MAKHNEVA, Petr SKLÁDAL, David NEČAS, Jan CECHAL et. al.

Basic information

Original name

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

Authors

MANAKHOV, Anton (643 Russian Federation, belonging to the institution), Ekaterina MAKHNEVA (643 Russian Federation, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), David NEČAS (203 Czech Republic, belonging to the institution), Jan CECHAL (203 Czech Republic), Lukas KALINA (203 Czech Republic), Marek ELIÁŠ (203 Czech Republic, belonging to the institution) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Applied Surface Science, AMSTERDAM, Elsevier Science BV, 2016, 0169-4332

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.387

RIV identification code

RIV/00216224:14740/16:00093848

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.apsusc.2015.10.178

UT WoS

000366591200005

Keywords in English

Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM

Abstract

V originále

The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

LM2011020, research and development project

Name: CEITEC ? open access

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MANAKHOV, Anton, Ekaterina MAKHNEVA, Petr SKLÁDAL, David NEČAS, Jan CECHAL, Lukas KALINA, Marek ELIÁŠ and Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. Applied Surface Science. AMSTERDAM: Elsevier Science BV, 2016, vol. 360, January, p. 28-36. ISSN 0169-4332. Available from: https://dx.doi.org/10.1016/j.apsusc.2015.10.178.

@article{1375041,
   author = {Manakhov, Anton and Makhneva, Ekaterina and Skládal, Petr and Nečas, David and Cechal, Jan and Kalina, Lukas and Eliáš, Marek and Zajíčková, Lenka},
   article_location = {AMSTERDAM},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.apsusc.2015.10.178},
   keywords = {Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM},
   language = {eng},
   issn = {0169-4332},
   journal = {Applied Surface Science},
   title = {The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry},
   url = {http://www.sciencedirect.com/science/article/pii/S0169433215025970},
   volume = {360},
   year = {2016}
}

TY  - JOUR
ID  - 1375041
AU  - Manakhov, Anton - Makhneva, Ekaterina - Skládal, Petr - Nečas, David - Cechal, Jan - Kalina, Lukas - Eliáš, Marek - Zajíčková, Lenka
PY  - 2016
TI  - The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry
JF  - Applied Surface Science
VL  - 360
IS  - January
SP  - 28-36
EP  - 28-36
PB  - Elsevier Science BV
SN  - 01694332
KW  - Amine coating
KW  - Sensors
KW  - Bio-immobilization
KW  - Plasma polymerization
KW  - Cyclopropylamine
KW  - XPS
KW  - QCM
UR  - http://www.sciencedirect.com/science/article/pii/S0169433215025970
L2  - http://www.sciencedirect.com/science/article/pii/S0169433215025970
N2  - The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
ER  -

MANAKHOV, Anton, Ekaterina MAKHNEVA, Petr SKLÁDAL, David NEČAS, Jan CECHAL, Lukas KALINA, Marek ELIÁŠ and Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. \textit{Applied Surface Science}. AMSTERDAM: Elsevier Science BV, 2016, vol.~360, January, p.~28-36. ISSN~0169-4332. Available from: https://dx.doi.org/10.1016/j.apsusc.2015.10.178.

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