| MANAKHOV, Anton, Ekaterina MAKHNEVA, Petr SKLÁDAL, David NEČAS, Jan CECHAL, Lukas KALINA, Marek ELIÁŠ and Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. Applied Surface Science. AMSTERDAM: Elsevier Science BV, 2016, vol. 360, January, p. 28-36. ISSN 0169-4332. Available from: https://dx.doi.org/10.1016/j.apsusc.2015.10.178. |
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@article{1375041,
author = {Manakhov, Anton and Makhneva, Ekaterina and Skládal, Petr and Nečas, David and Cechal, Jan and Kalina, Lukas and Eliáš, Marek and Zajíčková, Lenka},
article_location = {AMSTERDAM},
article_number = {January},
doi = {http://dx.doi.org/10.1016/j.apsusc.2015.10.178},
keywords = {Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM},
language = {eng},
issn = {0169-4332},
journal = {Applied Surface Science},
title = {The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry},
url = {http://www.sciencedirect.com/science/article/pii/S0169433215025970},
volume = {360},
year = {2016}
}
TY - JOUR
ID - 1375041
AU - Manakhov, Anton - Makhneva, Ekaterina - Skládal, Petr - Nečas, David - Cechal, Jan - Kalina, Lukas - Eliáš, Marek - Zajíčková, Lenka
PY - 2016
TI - The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry
JF - Applied Surface Science
VL - 360
IS - January
SP - 28-36
EP - 28-36
PB - Elsevier Science BV
SN - 01694332
KW - Amine coating
KW - Sensors
KW - Bio-immobilization
KW - Plasma polymerization
KW - Cyclopropylamine
KW - XPS
KW - QCM
UR - http://www.sciencedirect.com/science/article/pii/S0169433215025970
L2 - http://www.sciencedirect.com/science/article/pii/S0169433215025970
N2 - The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
ER -
MANAKHOV, Anton, Ekaterina MAKHNEVA, Petr SKLÁDAL, David NEČAS, Jan CECHAL, Lukas KALINA, Marek ELIÁŠ and Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. \textit{Applied Surface Science}. AMSTERDAM: Elsevier Science BV, 2016, vol.~360, January, p.~28-36. ISSN~0169-4332. Available from: https://dx.doi.org/10.1016/j.apsusc.2015.10.178.
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