Other formats:
BibTeX
LaTeX
RIS
@article{1375043, author = {Makhneva, Ekaterina and Manakhov, Anton and Skládal, Petr and Zajíčková, Lenka}, article_location = {LAUSANNE}, article_number = {March}, doi = {http://dx.doi.org/10.1016/j.surfcoat.2015.09.035}, keywords = {Plasma polymerization; Cyclopropylamine; Stable amine-rich films; Quartz crystal microbalance; Immunosensor; Label-free detection}, language = {eng}, issn = {0257-8972}, journal = {SURFACE & COATINGS TECHNOLOGY}, title = {Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions}, url = {http://www.sciencedirect.com/science/article/pii/S0257897215302747}, volume = {290}, year = {2016} }
TY - JOUR ID - 1375043 AU - Makhneva, Ekaterina - Manakhov, Anton - Skládal, Petr - Zajíčková, Lenka PY - 2016 TI - Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions JF - SURFACE & COATINGS TECHNOLOGY VL - 290 IS - March SP - 116-123 EP - 116-123 PB - ELSEVIER SCIENCE SA SN - 02578972 KW - Plasma polymerization KW - Cyclopropylamine KW - Stable amine-rich films KW - Quartz crystal microbalance KW - Immunosensor KW - Label-free detection UR - http://www.sciencedirect.com/science/article/pii/S0257897215302747 L2 - http://www.sciencedirect.com/science/article/pii/S0257897215302747 N2 - Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, immobilization of the biorecognition biomolecules onto the sensor surface is always required. Cyclopropylamine pulsed plastha polymerization in radio frequency (RF) capacitive discharges was employed to deposit amine thin films on the gold electrode of quartz crystal microbalance (QCM) biosensors and the monoclonal antibody AL-01, specific to human serum albumin (HSA), was immobilized onto the plasma polymer surface. Two different amine plasma polymers were studied. They had a similar amount of primary amine groups, 13-1.5 at.%, as determined by chemical derivatization but their stability in water was different. The 1st type, deposited at the floating potential and 120 Pa, exhibited 16% thickness loss after 24 h in water whereas the 2nd type deposited at RF electrode and 50 Pa was completely stable (2% thickness loss). Glutaraldehyde (GA) coupling of AL-01 was employed for both types of the amine films and the performance of QCM immunosensors was evaluated by the immunoassay flow test A high-stability of the frequency signal was obtained in both the cases but the 2nd type of the film provided low response to HSA. It was explained by its highly cross-linked structure and steric hindrance of active sites. Three different antibody immobilization methods were explored for the 1st type of the film. Stable baseline, selective and high response were recorded for GA and sulfo-SMCC methods. The results confirmed that the presented methodologies for the grafting of biomolecules on the gold are highly efficient and very promising for future use in biosensing. (C) 2015 Elsevier B.V. All rights reserved. ER -
MAKHNEVA, Ekaterina, Anton MANAKHOV, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions. \textit{SURFACE \&{} COATINGS TECHNOLOGY}. LAUSANNE: ELSEVIER SCIENCE SA, 2016, vol.~290, March, p.~116-123. ISSN~0257-8972. Available from: https://dx.doi.org/10.1016/j.surfcoat.2015.09.035.
|