Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis

MAZUR, Ewa, Eva BENKOVA and Jiří FRIML. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2016, vol. 6, September, p. nestránkováno, 15 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/srep33754.

Basic information

• Original name: Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis
• Authors: MAZUR, Ewa (616 Poland, guarantor, belonging to the institution), Eva BENKOVA (203 Czech Republic) and Jiří FRIML (203 Czech Republic).
• Edition: Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2016, 2045-2322.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.259
• RIV identification code: RIV/00216224:14740/16:00088767
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1038/srep33754
• UT WoS: 000383572400003
• Keywords in English: DEPENDENT AUXIN GRADIENTS; VEIN PATTERN-FORMATION; SECONDARY XYLEM; CIRCULAR VESSELS; THALIANA BRASSICACEAE; AXILLARY BUDS; PIN PROTEINS; SCOTS PINE; DIFFERENTIATION; PLANTS
• Tags: rivok

Abstract

Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• EE2.3.20.0043, research and development project: Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů
• GA13-39982S, research and development project: Name: Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese.

Investor: Czech Science Foundation

• GA13-40637S, research and development project: Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation